PMID- 15253669
OWN - NLM
STAT- MEDLINE
DCOM- 20050524
LR  - 20131121
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 76
IP  - 14
DP  - 2004 Jul 15
TI  - Development of a membrane-based immunofiltration assay for the detection of T-2 
      toxin.
PG  - 4237-40
AB  - An improved analytical device capable of performing simultaneous 
      immunofiltration-based immunoassay on 30 samples in the presence of reference 
      standards has been developed. The device consists of a rectangular membrane with 
      36 antibody spotted zones, one end of which was attached to a semirigid 
      polyethylene card. A piece of wetted filter paper between the membrane and the 
      polyethylene card absorbs the added reagent. The assay is a competitive one using 
      T-2 toxin-horseradish peroxidase (T-2 toxin-HRP) as the labeled analyte and 
      4-chloro-1 naphthol (4CN) as the substrate. Signal amplification was done by the 
      Super-CARD signal amplification method. Semiquantitative results were obtained by 
      visual comparison of the color intensity of a sample spot with those of reference 
      standards. Densitometric analysis was used for quantitation. The method allows 
      rapid and easy determination of T-2 toxin in wheat and poultry feed with 
      detection limits of 12.5 and 25 microg x kg(-)(1), respectively, with accuracy 
      and precision. Matrix interference was eliminated by appropriate dilution of 
      sample extracts with assay buffer. The detection sensitivity in ELISA was 10-fold 
      higher than that in the membrane-based method. Noninfected samples were spiked 
      with T-2 toxin at several concentrations and analyzed by the present method and 
      rapid ELISA. Mean recoveries by both methods were between 80 and 108%. The 
      correlation between the two methods was excellent (R(2) = 0.99).
FAU - Pal, Arindam
AU  - Pal A
AD  - Department of Immunobiology, Indian Institute of Chemical Biology, 4, Raja S C 
      Mullick Road, Jadavpur, Calcutta 700 032, India.
FAU - Acharya, Debopam
AU  - Acharya D
FAU - Saha, Debjani
AU  - Saha D
FAU - Dhar, Tarun K
AU  - Dhar TK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - I3FL5NM3MO (T-2 Toxin)
SB  - IM
MH  - Animal Feed
MH  - Enzyme-Linked Immunosorbent Assay/methods
MH  - Immunoassay/*methods
MH  - Membranes/metabolism
MH  - T-2 Toxin/*analysis/metabolism
MH  - Triticum/chemistry
EDAT- 2004/07/16 05:00
MHDA- 2005/05/25 09:00
CRDT- 2004/07/16 05:00
PHST- 2004/07/16 05:00 [pubmed]
PHST- 2005/05/25 09:00 [medline]
PHST- 2004/07/16 05:00 [entrez]
AID - 10.1021/ac049631s [doi]
PST - ppublish
SO  - Anal Chem. 2004 Jul 15;76(14):4237-40. doi: 10.1021/ac049631s.