PMID- 15253719
OWN - NLM
STAT- MEDLINE
DCOM- 20041101
LR  - 20061115
IS  - 0085-2538 (Print)
IS  - 0085-2538 (Linking)
VI  - 66
IP  - 2
DP  - 2004 Aug
TI  - Proximal tubule cells stimulated by lipopolysaccharide inhibit macrophage 
      activation.
PG  - 655-62
AB  - BACKGROUND: Tubule cells can produce a variety of cytokines, extracellular matrix 
      (ECM) components, and adhesion molecules in vitro and in vivo. It is generally 
      assumed that stimulated tubule cells are proinflammatory and at least partially 
      responsible for interstitial inflammation. However, the overall effect of tubular 
      cells on interstitial cells is unknown. In this study, pro- and anti-inflammatory 
      cytokine production and net effects on macrophages of tubule cells activated by 
      lipopolysaccharide (LPS) were examined. METHODS: Tubule cells stimulated with LPS 
      expressed tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-1beta, IL-12, 
      monocyte chemoattractant protein-1 (MCP-1), IL-10, and transforming growth 
      factor-beta (TGF-beta). Conditioned media were collected from confluent 
      monolayers of rat tubule cells stimulated, or not, by LPS for 4 and 18 hours, 
      respectively. Macrophages were cultured with conditioned media and/or LPS (0.5 
      microg/mL) for 18 hours. RESULTS: TNF-alpha and IL-lbeta mRNA of macrophages 
      stimulated by LPS increased more than fivefold when cultured with control 
      conditioned media from unstimulated tubule cells. Surprisingly, TNF-alpha and 
      IL-lbeta levels of macrophages stimulated by LPS were not increased when cultured 
      with conditioned media from activated tubule cells. Neutralizing antibodies to 
      IL-10 and TGF-beta were used to define the inhibitory component(s) in conditioned 
      medium. Anti-IL-10, but not anti-TGF-beta, abolished partially the inhibitory 
      effects of conditioned media on macrophages. CONCLUSION: Tubule cells produce 
      both pro- and anti-inflammatory cytokines and the net effect, partially explained 
      by IL-10, of tubule cells activated with LPS is to inhibit activity of 
      macrophages. Thus, the net effect of activated tubule cells on interstitial 
      pathology may in certain circumstances, be anti- rather than pro-inflammatory.
FAU - Wang, Yiping
AU  - Wang Y
AD  - Center for Transplantation and Renal Research, The University of Sydney at 
      Westmead Millenium Institute,Westmead, Sydney, Australia. 
      Yiping_Wang@wsahs.nsw.gov.au
FAU - Tay, Yuet-Ching
AU  - Tay YC
FAU - Harris, David C H
AU  - Harris DC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Animals
MH  - Cell Communication/immunology
MH  - Cells, Cultured
MH  - Culture Media, Conditioned/pharmacology
MH  - Cytokines/genetics
MH  - Gene Expression/immunology
MH  - Kidney Tubules, Proximal/*cytology/*drug effects/immunology
MH  - Lipopolysaccharides/*pharmacology
MH  - Macrophages/*cytology/*immunology
MH  - Male
MH  - Paracrine Communication/immunology
MH  - Rats
MH  - Rats, Wistar
EDAT- 2004/07/16 05:00
MHDA- 2004/11/02 09:00
CRDT- 2004/07/16 05:00
PHST- 2004/07/16 05:00 [pubmed]
PHST- 2004/11/02 09:00 [medline]
PHST- 2004/07/16 05:00 [entrez]
AID - S0085-2538(15)50094-X [pii]
AID - 10.1111/j.1523-1755.2004.00786.x [doi]
PST - ppublish
SO  - Kidney Int. 2004 Aug;66(2):655-62. doi: 10.1111/j.1523-1755.2004.00786.x.