PMID- 15253858
OWN - NLM
STAT- MEDLINE
DCOM- 20041119
LR  - 20181130
IS  - 1075-5535 (Print)
IS  - 1075-5535 (Linking)
VI  - 10
IP  - 3
DP  - 2004 Jun
TI  - Asian pear pectin administration during presensitization inhibits allergic 
      response to ovalbumin in BALB/c mice.
PG  - 527-34
AB  - OBJECTIVE: A type of respiratory disorder resembling some aspects of human 
      allergic asthma can be induced in mice using ovalbumin. The factors that 
      influence the etiology of asthma are poorly understood even though cytokines are 
      known to play a pivotal role. The purpose of this study was to test the 
      hypothesis whether an administration of Asian pear pectin during presensitization 
      could suppress allergic response to ovalbumin in BALB/c mice. DESIGN: High-dose 
      (100 microg) of pectin-sol was used and values were compared to those from the 
      control. Ovalbumin and aluminum hydroxide were utilized for sensitization while 
      ovalbumin aerosol was used for provocation 2 weeks later. The bronchoalveolar 
      lavage (BAL) and assessment of tracheal smooth muscle responsiveness to 
      electrical field stimulation or acetylcholine were performed 1 day after 
      ovalbumin provocation. Two main cytokines of interferon (IFN)-gamma and 
      interleukin (IL)-5, and serum immunoglobulin E (IgE) were assayed. SETTINGS: 
      Laboratory of the Chosun University Medical School SUBJECT: Male BALB/c mice 
      RESULTS: Antigen dose of 5 microg for sensitization generated TH1 type cytokines 
      in the lungs with a high level of IFN-gamma and a low level of IL-5. In contrast, 
      TH2 type cytokines were produced in splenocytes including a high level of IL-5 
      and a low level of IFN-gamma. Asian pear pectin-sol administration during 
      presensitization significantly inhibited (p < 0.05) sensitivity of airway smooth 
      muscle to electrical field stimulation and acetylcholine. Further, IFN-gamma 
      production significantly decreased (p < 0.05) in BAL fluids while it 
      significantly increased (p < 0.05) in splenic cells. On the other hand, IL-5 
      production significantly increased (p < 0.05) in BAL fluids while it was a 
      significant decrease (p < 0.05) in splenic cells. For the histopathologic changes 
      in the lung, pear pectin-sol recovered ovalbumin (OVA)-induced abnormal signs to 
      an almost normal state. As a correlate, IgE production significantly decreased (p 
      < 0.05) in pectin-sol-treated animals compared to the control. CONCLUSIONS: It is 
      possible from these data that BALB/c mice have different susceptibilities to 
      different doses of OVA regulated by pulmonary TH1 and TH2 type cytokines, 
      independent of splenic TH1 and TH2 type cytokines production. These results also 
      indicate that administration of Asian pear pectin-sol in presensitized mice 
      suppresses allergic asthmatic reaction.
CI  - Copyright Mary Ann Liebert, Inc.
FAU - Lee, Jae Chang
AU  - Lee JC
AD  - Department of Dental Hygiene, Naju College, Naju, Korea.
FAU - Pak, Sok Cheon
AU  - Pak SC
FAU - Lee, Seung Hoo
AU  - Lee SH
FAU - Na, Chang Su
AU  - Na CS
FAU - Lim, Sung Chul
AU  - Lim SC
FAU - Song, Chang Hun
AU  - Song CH
FAU - Bai, Young Hoon
AU  - Bai YH
FAU - Jang, Chul Ho
AU  - Jang CH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Altern Complement Med
JT  - Journal of alternative and complementary medicine (New York, N.Y.)
JID - 9508124
RN  - 0 (Allergens)
RN  - 0 (Interleukin-18)
RN  - 0 (Interleukin-5)
RN  - 0 (Plant Extracts)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 5QB0T2IUN0 (Aluminum Hydroxide)
RN  - 89NA02M4RX (Pectins)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Allergens/administration & dosage/*drug effects
MH  - Aluminum Hydroxide
MH  - Animals
MH  - Asthma/chemically induced/*drug therapy/prevention & control
MH  - Bronchoalveolar Lavage Fluid/immunology
MH  - Disease Models, Animal
MH  - Dose-Response Relationship, Immunologic
MH  - Immunoglobulin E/blood
MH  - Interleukin-18/blood
MH  - Interleukin-5/blood
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Ovalbumin/administration & dosage/*antagonists & inhibitors
MH  - Pectins/administration & dosage/*pharmacology
MH  - *Phytotherapy
MH  - Plant Extracts/administration & dosage/*pharmacology
MH  - Pulmonary Alveoli/drug effects/immunology
MH  - *Pyrus
MH  - Th1 Cells/immunology
MH  - Th2 Cells/immunology
EDAT- 2004/07/16 05:00
MHDA- 2004/12/16 09:00
CRDT- 2004/07/16 05:00
PHST- 2004/07/16 05:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/07/16 05:00 [entrez]
AID - 10.1089/1075553041323867 [doi]
PST - ppublish
SO  - J Altern Complement Med. 2004 Jun;10(3):527-34. doi: 10.1089/1075553041323867.