PMID- 15255305
OWN - NLM
STAT- MEDLINE
DCOM- 20040819
LR  - 20131121
IS  - 0940-9602 (Print)
IS  - 0940-9602 (Linking)
VI  - 186
IP  - 3
DP  - 2004 Jun
TI  - Low-dose radiation modifies the progression of chronic renal failure.
PG  - 277-82
AB  - Previous studies have indicated that the application of low dose radiation to an 
      arterial ligation has the potential to subsequently reduce or eliminate 
      restenosis caused by smooth muscle cell proliferation. Sufficient kidney 
      irradiation causes a radiation nephropathy and often leads to renal failure. In 
      order to evaluate the effect of low-dose irradiation on the kidney we 
      hypothesized that this particular therapy modifies renal injury in rats with 
      renal ablation and subsequently slows the rate of the progression. For further 
      clarification of the effect of irradiation at low doses, we determined 
      proliferating cell nuclear antigen (PCNA) and monocyte chemoattractant protein-1 
      (MCP-1) expression in remnant kidneys after low-dose radiation. Adult Wistar rats 
      (n = 10) were studied during the two weeks after renal ablation. The left kidney 
      was irradiated 24 hours after an operation in anaesthetised animals with 3 Grey 
      in a single dose. Ablated rats without irradiation (n = 9) served as 
      nephrectomized animals group. Rats without surgery and without radiation (n = 10) 
      served as healthy controls. Renal damage was assessed using the following 
      parameters: urine protein excretion rate (UprotV, mg/day), awake systolic blood 
      pressure (SBP, mm Hg), serum creatinine (SCr, micromol/l). The indirect 
      immunofluorescence method was used for the detection of PCNA and MCP-1 
      expression. Glomerular and tubular immunostaining was scored semiquantitatively. 
      Numerous PCNA positive cells and MCP-1 expression were present in the glomerulus 
      and tubulointerstitium in nephrectomized rat kidneys. Low-dose radiation 
      application was associated with a significant reduction in PCNA and low MCP-1 
      expression. This study shows that the application of low-dose irradiation has the 
      potential to modify the progression of chronic renal failure in rats.
FAU - Aunapuu, Marina
AU  - Aunapuu M
AD  - Department of Anatomy, University of Tartu, Ravila 19, 51014 Tartu, Estonia. 
      mariaun@ut.ee
FAU - Pechter, Ulle
AU  - Pechter U
FAU - Gerskevits, Eduard
AU  - Gerskevits E
FAU - Marjamagi, Margit-Maie
AU  - Marjamagi MM
FAU - Suuroja, Sirje
AU  - Suuroja S
FAU - Arend, Andres
AU  - Arend A
FAU - Kolts, Ivo
AU  - Kolts I
FAU - Kuhnel, Wolfgang
AU  - Kuhnel W
FAU - Ots, Mai
AU  - Ots M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Ann Anat
JT  - Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische 
      Gesellschaft
JID - 100963897
RN  - 0 (Chemokine CCL2)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - AYI8EX34EU (Creatinine)
SB  - IM
MH  - Animals
MH  - Blood Pressure/radiation effects
MH  - Body Weight
MH  - Chemokine CCL2/analysis
MH  - Creatinine/blood
MH  - Kidney/*anatomy & histology/*radiation effects
MH  - Kidney Failure, Chronic/*radiotherapy
MH  - Nephrectomy
MH  - Organ Size
MH  - Proliferating Cell Nuclear Antigen/analysis
MH  - Proteinuria
MH  - Rats
MH  - Rats, Wistar
EDAT- 2004/07/17 05:00
MHDA- 2004/08/20 05:00
CRDT- 2004/07/17 05:00
PHST- 2004/07/17 05:00 [pubmed]
PHST- 2004/08/20 05:00 [medline]
PHST- 2004/07/17 05:00 [entrez]
AID - S0940-9602(04)80017-7 [pii]
AID - 10.1016/S0940-9602(04)80017-7 [doi]
PST - ppublish
SO  - Ann Anat. 2004 Jun;186(3):277-82. doi: 10.1016/S0940-9602(04)80017-7.