PMID- 15256063
OWN - NLM
STAT- MEDLINE
DCOM- 20050412
LR  - 20181130
IS  - 1522-8002 (Print)
IS  - 1476-5586 (Electronic)
IS  - 1476-5586 (Linking)
VI  - 6
IP  - 4
DP  - 2004 Jul-Aug
TI  - Aberrant gene expression in human non small cell lung carcinoma cells exposed to 
      demethylating agent 5-aza-2'-deoxycytidine.
PG  - 412-9
AB  - The identification of genes undergoing genetic or epigenetic alterations and 
      contributing to the development of cancer is critical to our understanding of the 
      molecular mechanisms of carcinogenesis. A new approach in identifying alterations 
      of genes that might be relevant to the process of tumor development was used in 
      this study by examining the gene expression profile in human lung cancer cells 
      exposed to 5-aza-2'-deoxycytidine (5-aza-dC). A cDNA array analysis was carried 
      out on 5-aza-dC-treated and untreated non small cell lung cancer (NSCLC) cell 
      line NCI-H522. Sixteen and 14 genes were upregulated and downregulated, 
      respectively, by 5-aza-dC treatment. Among them, downregulation of tyrosine 
      protein kinase ABL2 (ABL2) gene and upregulation of hint/protein kinase C 
      inhibitor 1 (Hint/PKCI-1), DVL1, TIMP-1, and TRP-1 genes were found in expanded 
      observations in two or three of five 5-aza-dC-treated NSCLC cell lines. Among 
      these genes, we found that cDNA transfer of Hint/PKCI-1 resulted in a significant 
      in vitro growth inhibition in two cell lines exhibiting 5-aza-dC-induced 
      upregulation of Hint/PKCI-1 and significantly reduced in vivo tumorigenicity of 
      one NSCLC cell line. Hint/PKCI-1, which is the only other characterized human 
      histidine triad (HIT) nucleotide-binding protein in addition to tumor-suppressor 
      gene FHIT, might be involved in lung carcinogenesis.
CI  - Copyright 2004 Neoplasia Press, Inc.
FAU - Yuan, Bao-Zhu
AU  - Yuan BZ
AD  - Laboratory of Cancer Genetics, Toxicology and Molecular Biology Branch, National 
      Institute for Occupational Safety and Health, 1095 Willowdale Road, Morgantown, 
      WV 26505, USA. bby1@cdc.gov
FAU - Jefferson, Amy M
AU  - Jefferson AM
FAU - Popescu, Nicholas C
AU  - Popescu NC
FAU - Reynolds, Steven H
AU  - Reynolds SH
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Neoplasia
JT  - Neoplasia (New York, N.Y.)
JID - 100886622
RN  - 0 (Antimetabolites, Antineoplastic)
RN  - 776B62CQ27 (Decitabine)
RN  - M801H13NRU (Azacitidine)
SB  - IM
MH  - Antimetabolites, Antineoplastic/toxicity
MH  - Apoptosis/drug effects
MH  - Azacitidine/*analogs & derivatives/*toxicity
MH  - Carcinoma, Non-Small-Cell Lung
MH  - Cell Division/drug effects
MH  - Cell Line, Tumor
MH  - DNA Methylation
MH  - Decitabine
MH  - Gene Expression Regulation, Neoplastic/*drug effects
MH  - Humans
MH  - Kinetics
MH  - Lung Neoplasms
MH  - Oligonucleotide Array Sequence Analysis
MH  - Transfection
PMC - PMC1502114
EDAT- 2004/07/17 05:00
MHDA- 2005/04/13 09:00
PMCR- 2004/07/01
CRDT- 2004/07/17 05:00
PHST- 2004/07/17 05:00 [pubmed]
PHST- 2005/04/13 09:00 [medline]
PHST- 2004/07/17 05:00 [entrez]
PHST- 2004/07/01 00:00 [pmc-release]
AID - 03490 [pii]
AID - 10.1593/neo.03490 [doi]
PST - ppublish
SO  - Neoplasia. 2004 Jul-Aug;6(4):412-9. doi: 10.1593/neo.03490.