PMID- 15257086
OWN - NLM
STAT- MEDLINE
DCOM- 20050131
LR  - 20190917
IS  - 1073-2322 (Print)
IS  - 1073-2322 (Linking)
VI  - 22
IP  - 2
DP  - 2004 Aug
TI  - Polymicrobial sepsis induces divergent effects on splenic and peritoneal 
      dendritic cell function in mice.
PG  - 137-44
AB  - Dendritic cells (DCs) are professional antigen-presenting cells that act as 
      sentinels in the cell-mediated response against invading pathogens associated 
      with septic challenge. The purpose of the present study was to determine whether 
      there is a loss of dendritic cells and/or changes in function of these cells in 
      septic mice. Here we report that the number of DCs, in both spleen and 
      peritoneum, decreased over 24 h postsepsis [cecal ligation and puncture (CLP)] 
      when compared with sham. The most dramatic change was seen in the peritoneal 
      cavity. This decrease appeared to be caused mainly by the depletion of immature 
      DCs rather than mature DCs. This change was LPS independent and minimally 
      affected by FasL; however, overexpression of human Bcl-2 gene provides protection 
      of the septic peritoneal DCs. Moreover, although the level of IL-12 release 
      decreased significantly in splenic DCs obtained from CLP mice, IL-12 secretion 
      was markedly elevated by peritoneal DCs as well as in both plasma and peritoneal 
      fluid at 24 h post-CLP. In peritoneal cells, the expression of CD40, CD80, and 
      CD86 was unchanged, but their respective ligands CD40L, CD28, and CD152 all 
      increased in mice 24 h after CLP, although no such change was observed in 
      splenocytes. Regardless of the presence or absence of antigen, peritoneal DCs 
      from CLP mice showed higher capacity to stimulate T-cell proliferation than those 
      cells from the sham control. However, splenic DCs from CLP mice only showed 
      augmented capacity to induce antigen-dependent stimulation of T-cell 
      proliferation. Together, these data indicate that sepsis produces divergent 
      functional changes in splenic and peritoneal DC populations.
FAU - Ding, Yanli
AU  - Ding Y
AD  - Division of Surgical Research, Department of Surgery, Rhode Island Hospital, 
      Brown University School of Medicine, Providence, Rhode Island 02903, USA.
FAU - Chung, Chun-Shiang
AU  - Chung CS
FAU - Newton, Sarah
AU  - Newton S
FAU - Chen, Yaping
AU  - Chen Y
FAU - Carlton, Stacey
AU  - Carlton S
FAU - Albina, Jorge E
AU  - Albina JE
FAU - Ayala, Alfred
AU  - Ayala A
LA  - eng
GR  - R01 GM046354/GM/NIGMS NIH HHS/United States
GR  - R01 GM046354-12/GM/NIGMS NIH HHS/United States
GR  - R01 HL073525/HL/NHLBI NIH HHS/United States
GR  - R01-GM46354/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Shock
JT  - Shock (Augusta, Ga.)
JID - 9421564
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (B7-1 Antigen)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD11c Antigen)
RN  - 0 (CD40 Antigens)
RN  - 0 (CTLA-4 Antigen)
RN  - 0 (CTLA4 protein, human)
RN  - 0 (Cd86 protein, mouse)
RN  - 0 (Ctla4 protein, mouse)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (FASLG protein, human)
RN  - 0 (Fas Ligand Protein)
RN  - 0 (Fasl protein, mouse)
RN  - 0 (Ligands)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Tlr4 protein, mouse)
RN  - 0 (Toll-Like Receptor 4)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Animals
MH  - Antigen Presentation
MH  - Antigens, CD/biosynthesis
MH  - Antigens, Differentiation/biosynthesis
MH  - B7-1 Antigen/biosynthesis
MH  - B7-2 Antigen
MH  - CD11c Antigen/biosynthesis
MH  - CD40 Antigens/biosynthesis
MH  - CTLA-4 Antigen
MH  - Culture Media, Conditioned/pharmacology
MH  - Dendritic Cells/*cytology/metabolism/microbiology
MH  - Fas Ligand Protein
MH  - Flow Cytometry
MH  - Interleukin-12/blood/metabolism
MH  - Ligands
MH  - Lipopolysaccharides/metabolism
MH  - Male
MH  - Membrane Glycoproteins/biosynthesis/metabolism
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Peritoneal Lavage
MH  - Peritoneum/metabolism
MH  - Phenotype
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
MH  - Receptors, Cell Surface/metabolism
MH  - Sepsis/*microbiology
MH  - Spleen/*cytology/microbiology
MH  - Time Factors
MH  - Toll-Like Receptor 4
PMC - PMC2253681
MID - NIHMS40047
EDAT- 2004/07/17 05:00
MHDA- 2005/02/03 09:00
PMCR- 2008/02/24
CRDT- 2004/07/17 05:00
PHST- 2004/07/17 05:00 [pubmed]
PHST- 2005/02/03 09:00 [medline]
PHST- 2004/07/17 05:00 [entrez]
PHST- 2008/02/24 00:00 [pmc-release]
AID - 00024382-200408000-00007 [pii]
AID - 10.1097/01.shk.0000131194.80038.3f [doi]
PST - ppublish
SO  - Shock. 2004 Aug;22(2):137-44. doi: 10.1097/01.shk.0000131194.80038.3f.