PMID- 15257465
OWN - NLM
STAT- MEDLINE
DCOM- 20050302
LR  - 20181113
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 113
IP  - 1
DP  - 2004 Aug
TI  - Diverse patterns of the tandem repeats organization in rye chromosomes.
PG  - 42-52
AB  - Although the monomer size, nucleotide sequence, abundance and species 
      distribution of tandemly organized DNA families are well characterized, little is 
      known about the internal structure of tandem arrays, including total arrays size 
      and the pattern of monomers distribution. Using our rye specific probes, pSc200 
      and pSc250, we addressed these issues for telomere associated rye heterochromatin 
      where these families are very abundant. Fluorescence in situ hybridization (FISH) 
      on meiotic chromosomes revealed a specific mosaic arrangement of domains for each 
      chromosome arm where either pSc200 or pSc250 predominates without any obvious 
      tendency in order and size of domains. DNA of rye-wheat monosomic additions 
      studied by pulse field gel electrophoresis produced a unique overall blot 
      hybridization display for each of the rye chromosomes. The FISH signals on DNA 
      fibres showed multiple monomer arrangement patterns of both repetitive families 
      as well as of the Arabidopsis-type telomere repeat. The majority of the arrays 
      consisted of the monomers of both families in different patterns separated by 
      spacers. The primary structure of some spacer sequences revealed scrambled 
      regions of similarity to various known repetitive elements. This level of 
      complexity in the long-range organization of tandem arrays has not been 
      previously reported for any plant species. The various patterns of internal 
      structure of the tandem arrays are likely to have resulted from evolutionary 
      interplay, array homogenization and the generation of heterogeneity mediated by 
      double-strand breaks and associated repair mechanisms.
FAU - Alkhimova, Olena G
AU  - Alkhimova OG
AD  - Institute of Molecular Biology and Genetics, Kiev 03143, Ukraine.
FAU - Mazurok, Nina A
AU  - Mazurok NA
FAU - Potapova, Tatyana A
AU  - Potapova TA
FAU - Zakian, Suren M
AU  - Zakian SM
FAU - Heslop-Harrison, John S
AU  - Heslop-Harrison JS
FAU - Vershinin, Alexander V
AU  - Vershinin AV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040715
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
SB  - IM
MH  - *Chromosomes, Plant
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - In Situ Hybridization, Fluorescence
MH  - Secale/*genetics
MH  - *Tandem Repeat Sequences
EDAT- 2004/07/17 05:00
MHDA- 2005/03/03 09:00
CRDT- 2004/07/17 05:00
PHST- 2004/02/19 00:00 [received]
PHST- 2004/04/20 00:00 [revised]
PHST- 2004/05/14 00:00 [accepted]
PHST- 2004/07/17 05:00 [pubmed]
PHST- 2005/03/03 09:00 [medline]
PHST- 2004/07/17 05:00 [entrez]
AID - 10.1007/s00412-004-0294-4 [doi]
PST - ppublish
SO  - Chromosoma. 2004 Aug;113(1):42-52. doi: 10.1007/s00412-004-0294-4. Epub 2004 Jul 
      15.