PMID- 15258806
OWN - NLM
STAT- MEDLINE
DCOM- 20050429
LR  - 20181113
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 113
IP  - 2
DP  - 2004 Sep
TI  - Genomic instability in both wild-type and telomerase null MEFs.
PG  - 62-8
AB  - To examine chromosome instability in the absence of telomerase, we established 
      mouse embryonic fibroblast (MEF) lines from late generation mTR-/- and wild-type 
      animals and examined metaphases using telomere fluorescence in situ hybridization 
      (FISH) and spectral karyotyping (SKY). In early passages, mTR-/- G6 cell lines 
      showed more chromosome ends with no telomere signal, more chromosome end-to-end 
      fusions and greater radiosensitivity than wild-type lines. At later passages, 
      however, the rate of genomic instability in the wild-type MEFs increased to a 
      level similar or higher than seen in the mTR-/- G6 cell lines. This high degree 
      of instability in wild-type MEF lines suggests that post-crisis MEFs should not 
      be considered genetically defined cell lines. Surprisingly, the increased 
      radiosensitivity seen in early passage mTR-/- G6 cultures was lost after crisis. 
      Both post-crisis mTR-/- G6 MEFs and wild-type MEFs showed loss of p53 and 
      gamma-H2AX phosphorylation in response to irradiation, indicating a loss of DNA 
      damage checkpoints.
FAU - Hao, Ling-Yang
AU  - Hao LY
AD  - Graduate Program in Human Genetics, Department of Molecular Biology and Genetics, 
      Johns Hopkins University School of Medicine, 725 N Wolfe Street, Baltimore, MD 
      21205, USA.
FAU - Greider, Carol W
AU  - Greider CW
LA  - eng
GR  - CA16519/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20040716
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
RN  - 0 (Histones)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 0 (gamma-H2AX protein, mouse)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - DNA Damage/genetics/physiology/radiation effects
MH  - Embryo, Mammalian/cytology/*physiology
MH  - Fibroblasts/*physiology
MH  - Gamma Rays
MH  - Gene Deletion
MH  - Genomic Instability/genetics/*physiology/radiation effects
MH  - Histones/genetics
MH  - Mice
MH  - Mice, Knockout
MH  - Radiation Tolerance/genetics/*physiology/radiation effects
MH  - Telomere/genetics/metabolism
MH  - Tumor Suppressor Protein p53/genetics/metabolism
EDAT- 2004/07/20 05:00
MHDA- 2005/04/30 09:00
CRDT- 2004/07/20 05:00
PHST- 2004/03/25 00:00 [received]
PHST- 2004/05/06 00:00 [revised]
PHST- 2004/05/09 00:00 [accepted]
PHST- 2004/07/20 05:00 [pubmed]
PHST- 2005/04/30 09:00 [medline]
PHST- 2004/07/20 05:00 [entrez]
AID - 10.1007/s00412-004-0291-7 [doi]
PST - ppublish
SO  - Chromosoma. 2004 Sep;113(2):62-8. doi: 10.1007/s00412-004-0291-7. Epub 2004 Jul 
      16.