PMID- 15260347
OWN - NLM
STAT- MEDLINE
DCOM- 20041005
LR  - 20190714
IS  - 0013-936X (Print)
IS  - 0013-936X (Linking)
VI  - 38
IP  - 12
DP  - 2004 Jun 15
TI  - MtBE biodegradation in a gravity flow, high-biomass retaining bioreactor.
PG  - 3449-56
AB  - The aerobic biodegradation of methyl tert-butyl ether (MtBE), a widely used fuel 
      oxygenate, was investigated using a pilot-scale biomass-retaining bioreactor 
      called a Biomass Concentrator Reactor (BCR). The reactor was operated for a year 
      at a flow rate of 2500 L/d on Cincinnati dechlorinated tap water and an influent 
      MtBE concentration of 5 mg/L. Treatment efficiency of MtBE in the reactor during 
      stable operations exceeded 99.9%. The upper 95% confidence levels of effluent 
      MtBE concentrations and its degradation byproduct tert-butyl alcohol (TBA) were 
      2.9 and 0.9 microg/L, respectively, during these stable conditions. In addition, 
      the effluent was found to be of better quality than the influent tap water as 
      reflected by dissolved organic carbon analysis. Microbial community DNA profiling 
      was carried out using denaturing gradient gel electrophoresis (DGGE) of 
      polymerase chain reaction amplified 16s rDNA. The BCR was found to be inhabited 
      by a wide spectrum of bacterial species, most notably microorganisms related to 
      the genera Hydrogenophaga, Methylobacterium, Sphingomonas, and Pseudomonas. These 
      organisms were previously reported to be associated with MtBE degradation. With 
      the contamination of groundwater by MtBE being a wide-ranging problem throughout 
      the United States, it is essential to develop a technology capable of effectively 
      remediating such aquifers in order to protect public health and the environment. 
      The BCR's simple operation and low maintenance requirements may render it an 
      economically attractive approach to remediating groundwater contaminated with 
      MtBE.
FAU - Zein, Maher M
AU  - Zein MM
AD  - Department of Civil and Environmental Engineering, University of Cincinnati, 
      Cincinnati, Ohio 45221, USA.
FAU - Suidan, Makram T
AU  - Suidan MT
FAU - Venosa, Albert D
AU  - Venosa AD
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Environ Sci Technol
JT  - Environmental science & technology
JID - 0213155
RN  - 0 (Carcinogens)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Methyl Ethers)
RN  - 29I4YB3S89 (methyl tert-butyl ether)
SB  - IM
MH  - Bacteria, Aerobic
MH  - Biodegradation, Environmental
MH  - Biomass
MH  - *Bioreactors
MH  - Carcinogens/*metabolism
MH  - DNA, Bacterial/analysis
MH  - Methyl Ethers/*metabolism
MH  - Polymerase Chain Reaction
MH  - Population Dynamics
MH  - Public Health
EDAT- 2004/07/21 05:00
MHDA- 2004/10/06 09:00
CRDT- 2004/07/21 05:00
PHST- 2004/07/21 05:00 [pubmed]
PHST- 2004/10/06 09:00 [medline]
PHST- 2004/07/21 05:00 [entrez]
AID - 10.1021/es030652y [doi]
PST - ppublish
SO  - Environ Sci Technol. 2004 Jun 15;38(12):3449-56. doi: 10.1021/es030652y.