PMID- 15261308
OWN - NLM
STAT- MEDLINE
DCOM- 20040914
LR  - 20220227
IS  - 0960-0760 (Print)
IS  - 0960-0760 (Linking)
VI  - 91
IP  - 1-2
DP  - 2004 Jun
TI  - Regulation of the lipopolysaccharide signal transduction pathway by 
      17beta-estradiol in macrophage cells.
PG  - 59-66
AB  - We have previously shown that 17beta-estradiol (E2) prevents the activation of 
      brain macrophages, i.e. microglia cells, both in vitro and in vivo. Hormone 
      exerts this inhibitory effect by inhibiting pro-inflammatory gene expression. In 
      this study we further investigated on the molecular mechanism of E2 action in the 
      RAW 264.7 macrophage cell line. We show here that these cells express the 
      alpha-isoform of the estrogen receptor (ERalpha) and not ERbeta. Similarly to its 
      activity in brain macrophages, E2 is able to inhibit the activation program 
      induced by lipopolysaccharide (LPS) in RAW 264.7 cells, as shown by the 
      inhibitory effect of hormone on the morphological conversion and matrix 
      metalloproteinase-9 (MMP-9) expression induced by the endotoxin. In addition, we 
      demonstrate that hormone treatment is not associated with a reduction in the 
      steady-state expression of Toll-like receptor-4 (TLR-4) and CD14, two components 
      of the LPS receptor complex. Our results further confirm the anti-inflammatory 
      role of ERalpha in macrophages and propose that the mechanism of hormone action 
      on macrophage reactivity involves signaling molecules which are down-stream 
      effectors of the LPS membrane receptors.
FAU - Vegeto, Elisabetta
AU  - Vegeto E
AD  - Department of Pharmacological Sciences, Center of Excellence on Neurodegenerative 
      Diseases, University of Milan, via Balzaretti, 9 20133, Italy.
FAU - Ghisletti, Serena
AU  - Ghisletti S
FAU - Meda, Clara
AU  - Meda C
FAU - Etteri, Sabrina
AU  - Etteri S
FAU - Belcredito, Silvia
AU  - Belcredito S
FAU - Maggi, Adriana
AU  - Maggi A
LA  - eng
GR  - GP0127Y01/TI_/Telethon/Italy
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Steroid Biochem Mol Biol
JT  - The Journal of steroid biochemistry and molecular biology
JID - 9015483
RN  - 0 (DNA, Complementary)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (Estrogens)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Tlr4 protein, rat)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Toll-Like Receptors)
RN  - 4TI98Z838E (Estradiol)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Brain/metabolism
MH  - Cell Line
MH  - Cells, Cultured
MH  - DNA, Complementary/metabolism
MH  - Enzyme Activation
MH  - Estradiol/*metabolism
MH  - Estrogen Receptor alpha
MH  - Estrogen Receptor beta
MH  - Estrogens/metabolism
MH  - Gene Expression Regulation
MH  - Lipopolysaccharide Receptors/biosynthesis
MH  - Lipopolysaccharides/*biosynthesis
MH  - Macrophages/*metabolism
MH  - Matrix Metalloproteinase 9/biosynthesis
MH  - Membrane Glycoproteins/biosynthesis
MH  - Mice
MH  - Microglia/metabolism
MH  - Microscopy, Fluorescence
MH  - Polymerase Chain Reaction
MH  - Protein Isoforms
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Cell Surface/biosynthesis
MH  - Receptors, Estrogen/biosynthesis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Signal Transduction
MH  - Time Factors
MH  - Toll-Like Receptor 4
MH  - Toll-Like Receptors
EDAT- 2004/07/21 05:00
MHDA- 2004/09/15 05:00
CRDT- 2004/07/21 05:00
PHST- 2003/02/19 00:00 [received]
PHST- 2004/02/02 00:00 [accepted]
PHST- 2004/07/21 05:00 [pubmed]
PHST- 2004/09/15 05:00 [medline]
PHST- 2004/07/21 05:00 [entrez]
AID - S0960076004002110 [pii]
AID - 10.1016/j.jsbmb.2004.02.004 [doi]
PST - ppublish
SO  - J Steroid Biochem Mol Biol. 2004 Jun;91(1-2):59-66. doi: 
      10.1016/j.jsbmb.2004.02.004.