PMID- 15261463
OWN - NLM
STAT- MEDLINE
DCOM- 20041001
LR  - 20131121
IS  - 0161-5890 (Print)
IS  - 0161-5890 (Linking)
VI  - 41
IP  - 9
DP  - 2004 Jul
TI  - Threonine at position 6 is not essential for the immunosuppressive activity of 
      HLA-DQ(beta164-172)-hexapeptide.
PG  - 911-7
AB  - It has been previously found that the nonapeptide fragment of human leukocyte 
      antigen (HLA)-DQ molecule, located in the beta chain 164-172 with the 
      Thr-Pro-Gln-Arg-Gly-Asp-Val-Tyr-Thr sequence, suppresses the immune response. The 
      hexapeptide: Arg-Gly-Asp-Val-Tyr-Thr was the shortest fragment of HLA-DQ showing 
      both cellular and humoral immunosuppressive activity, while the analog deprived 
      of the last amino acid (Arg-Gly-Asp-Val-Tyr) showed very weak stimulatory 
      activity with respect to the humoral immune response. This suggested that the 
      threonine residue in the hexapeptide plays an essential role in 
      immunosuppression. In this study, the role of the side chain of threonine residue 
      was scrutinized in a series of synthetic analogs in which the Thr residue was 
      substituted by various amino acids, amides and methyl ester. The synthesized 
      peptides were evaluated for their immunosuppressive activity. Our results 
      indicate that the substitutions did not significantly affect the immunomodulatory 
      properties, revealing that the threonine side chain is not critical for the 
      immunosuppressive potency of the peptides. Interestingly, a simple analogue, 
      pentapeptide amide H-Arg-Gly-Asp-Val-Tyr-NH2 possessed high immunosuppressive 
      potency, comparable to that of cyclosporine.
FAU - Stefanowicz, Piotr
AU  - Stefanowicz P
AD  - Faculty of Chemistry, University of Wroclaw, ul. F. Joliot-Curie 14, 50-383 
      Wroclaw, Poland. stp@wchuwr.chem.uni.wroc.pl
FAU - Boratynski, Przemyslaw J
AU  - Boratynski PJ
FAU - Staszewska, Anna
AU  - Staszewska A
FAU - Wilczynski, Andrzej
AU  - Wilczynski A
FAU - Zimecki, Michal
AU  - Zimecki M
FAU - Szewczuk, Zbigniew
AU  - Szewczuk Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (Peptides)
RN  - 2ZD004190S (Threonine)
SB  - IM
MH  - Biological Assay
MH  - HLA-DQ Antigens/*genetics/immunology
MH  - Humans
MH  - Immune Tolerance/*genetics/immunology
MH  - Peptides/*genetics/immunology
MH  - Threonine/*genetics
EDAT- 2004/07/21 05:00
MHDA- 2004/10/02 05:00
CRDT- 2004/07/21 05:00
PHST- 2003/12/27 00:00 [received]
PHST- 2004/07/21 05:00 [pubmed]
PHST- 2004/10/02 05:00 [medline]
PHST- 2004/07/21 05:00 [entrez]
AID - S0161589004001403 [pii]
AID - 10.1016/j.molimm.2004.04.024 [doi]
PST - ppublish
SO  - Mol Immunol. 2004 Jul;41(9):911-7. doi: 10.1016/j.molimm.2004.04.024.