PMID- 15261692
OWN - NLM
STAT- MEDLINE
DCOM- 20040923
LR  - 20061115
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 101
IP  - 1-2
DP  - 2004 Sep
TI  - Induction of auto-anti-idiotypic antibodies specific for antibodies to matrix and 
      envelope glycoprotein from pigs experimentally infected with porcine reproductive 
      and respiratory syndrome virus.
PG  - 49-59
AB  - Auto-anti-idiotypic antibodies (Aab-2s) specific for antibodies against envelope 
      glycoprotein GP5 were previously identified in 10 of 12 pigs experimentally 
      infected with porcine reproductive and respiratory syndrome virus (PRRSV). In 
      this study, we report in addition the induction of Aab-2s specific for antibodies 
      against matrix (M) and envelope glycoprotein GP5 antigens in 38 of 47 pigs 
      experimentally infected with PRRSV. Two sets of Aab-2s were induced in the sera, 
      one at 21 days post-infection (DPI) and peaked on 35 DPI and another at 49 DPI 
      and peaked on 77 DPI. The Aab-2s induced at an early stage were from non-virus 
      carrier pigs as defined when they were euthanized on and after 77 DPI. In 
      contrast, the Aab-2s induced at a later stage were from virus carrier pigs. These 
      Aab-2s possessed the characteristics of internal image Ab-2s and recognized 
      shared idiotypes on swine and murine antibodies to the GP5 and M antigens of 
      PRRSV. This study further demonstrates that, during the antibody responses to 
      PRRSV infection, Aab-2s specific for both anti-M and anti-GP5 antibodies were 
      induced. More importantly, the early and late Aab-2s possessed different 
      idiotype-binding specificities. By detecting and characterizing these Aab-2s, it 
      may be possible to identify whether pigs infected with PRRSV will clear the virus 
      or become virus carriers.
FAU - Zhou, En-Min
AU  - Zhou EM
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, 2630 Veterinary Medicine Building, 
      Ames 50011, USA. ezhou@iastate.edu
FAU - Clavijo, Alfonso
AU  - Clavijo A
FAU - Jiang, Zhengjun
AU  - Jiang Z
FAU - Ameri-Mahabadi, Mehrdad
AU  - Ameri-Mahabadi M
FAU - Zimmerman, Jeffrey J
AU  - Zimmerman JJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Antibodies, Anti-Idiotypic)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Autoantibodies)
RN  - 0 (Immunoglobulin Idiotypes)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Matrix Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Animals
MH  - Antibodies, Anti-Idiotypic/*biosynthesis/blood/immunology
MH  - Antibodies, Viral/blood
MH  - Autoantibodies/*biosynthesis/blood/immunology
MH  - Carrier State/immunology/veterinary
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Female
MH  - Fluorescent Antibody Technique/veterinary
MH  - Immunoglobulin Idiotypes/immunology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Swine
MH  - Viral Envelope Proteins/*immunology
MH  - Viral Matrix Proteins/*immunology
EDAT- 2004/07/21 05:00
MHDA- 2004/09/24 05:00
CRDT- 2004/07/21 05:00
PHST- 2003/10/10 00:00 [received]
PHST- 2004/02/24 00:00 [revised]
PHST- 2004/03/23 00:00 [accepted]
PHST- 2004/07/21 05:00 [pubmed]
PHST- 2004/09/24 05:00 [medline]
PHST- 2004/07/21 05:00 [entrez]
AID - S0165242704001230 [pii]
AID - 10.1016/j.vetimm.2004.03.007 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2004 Sep;101(1-2):49-59. doi: 
      10.1016/j.vetimm.2004.03.007.