PMID- 15263792
OWN - NLM
STAT- MEDLINE
DCOM- 20041026
LR  - 20220318
IS  - 0042-1138 (Print)
IS  - 0042-1138 (Linking)
VI  - 73
IP  - 1
DP  - 2004
TI  - p53 gene codon 72 polymorphism but not tumor necrosis factor-alpha gene is 
      associated with prostate cancer.
PG  - 41-6
AB  - OBJECTIVE: A polymorphism of gene p53 codon 72 is associated with various cancer 
      formations. Tumor necrosis factor-alpha (TNF-alpha) one of the cytokines secreted 
      by macrophages in response to inflammation and is also related to cancer 
      formation. We aimed to evaluate the association between prostate cancer and the 
      polymorphisms of the TNF-alpha gene promoter -308 and p53 gene codon 72. PATIENTS 
      AND METHODS: In our study, a normal control group of 126 healthy people and 96 
      patients with prostate cancer were examined. The polymorphism (G/A) of TNF-alpha 
      gene was detected by polymerase chain reaction (PCR)-based restriction analysis 
      (Nco I endonuclease) and the polymorphism of p53 gene was detected by two PCRs 
      (one for proline and one for arginine form). RESULTS: There was a significant 
      difference in the distribution of codon 72 polymorphism the p53 gene between 
      prostate cancer patients and the normal controls (p < 0.001). The proline form of 
      p53 gene codon 72 was significantly higher than the arginine form, with an odds 
      ratio of 2.606 (95% CI = 1.052-6.455). This difference was also revealed in the 
      tumor staging (p = 0.035) as the proline form was significantly higher in the 
      metastasis group of prostate cancer. There were no statistical differences in the 
      distribution of -308 polymorphism of the TNF-alpha gene between cancer patients 
      and the control subjects (p = 1.0). CONCLUSION: Prostate cancer appears to be 
      associated with the p53 gene codon 72 polymorphisms, but not with the TNF-alpha 
      gene. The proline form of p53 gene codon 72 might be a more significant risk 
      factor for the development of metastasis than the arginine form.
CI  - Copyright 2004 S. Karger AG, Basel
FAU - Wu, Hsi-Chin
AU  - Wu HC
AD  - Department of Urology, China Medical College Hospital, Taichung, Taiwan.
FAU - Chang, Chao-Hsiang
AU  - Chang CH
FAU - Chen, Huey-Yi
AU  - Chen HY
FAU - Tsai, Fuu-Jen
AU  - Tsai FJ
FAU - Tsai, Jeffery J P
AU  - Tsai JJ
FAU - Chen, Wen-Chi
AU  - Chen WC
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Urol Int
JT  - Urologia internationalis
JID - 0417373
RN  - 0 (Codon)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Codon
MH  - Genes, p53/*genetics
MH  - Genotype
MH  - Homozygote
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - *Polymorphism, Genetic
MH  - Prostatic Neoplasms/*genetics
MH  - Tumor Necrosis Factor-alpha/*genetics
EDAT- 2004/07/21 05:00
MHDA- 2004/10/27 09:00
CRDT- 2004/07/21 05:00
PHST- 2003/01/27 00:00 [received]
PHST- 2003/08/27 00:00 [accepted]
PHST- 2004/07/21 05:00 [pubmed]
PHST- 2004/10/27 09:00 [medline]
PHST- 2004/07/21 05:00 [entrez]
AID - 78803 [pii]
AID - 10.1159/000078803 [doi]
PST - ppublish
SO  - Urol Int. 2004;73(1):41-6. doi: 10.1159/000078803.