PMID- 15264307
OWN - NLM
STAT- MEDLINE
DCOM- 20051207
LR  - 20200930
IS  - 1552-4973 (Print)
IS  - 1552-4973 (Linking)
VI  - 70
IP  - 2
DP  - 2004 Aug 15
TI  - Osteoblast-like cell response to bioactive composites-surface-topography and 
      composition effects.
PG  - 250-61
AB  - Two bioactive composites, containing 40 vol % filler in high-density polyethylene 
      (HDPE), were investigated to examine the effects of different filler compositions 
      and different surface patterning. The first composite, known as HAPEX, consists 
      of hydroxyapatite within HDPE, and the second composite, known as AWPEX, consists 
      of glass-ceramic apatite-wollastonite in HDPE. Surface topography effects at 5-50 
      and 100-150 microm were explored, with cell morphology analyzed with the use of 
      scanning electron microscopy and confocal laser scanning microscopy (CLSM). 
      Biochemical assays of adenosine triphosphate and alkaline phosphatase were used 
      to analyze osteoblast-like cell proliferation and differentiation. For both 
      composites, cell alignment was seen along grooves, pillars, and wells, with 
      preferential cell attachment to ceramic particles within the polymer matrices. 
      HAPEX showed significantly increased cell proliferation over AWPEX (P < 0.005). 
      However, greater cell differentiation occurred for AWPEX over HAPEX (P < 0.005). 
      Polishing significantly increased osteoblast-like cell response over as-cut 
      samples, but surface-topography changes above 50 microm had no consistent effect. 
      Smaller-scale features also showed no significant trend in terms of cell 
      proliferation, but did show significant differences in cell differentiation (P < 
      0.05). CLSM imaging of actin and vinculin localization within cells showed the 
      greatest change in comparison to polished surface controls for cells cultured on 
      samples with surface features below 50 microm. The fact that similar observations 
      were made for both HAPEX and AWPEX indicated that, for these experiments, the 
      effects of surface topography more strongly influenced cell response than 
      chemical composition.
CI  - Copyright 2004 Wiley Periodicals, Inc.
FAU - Rea, Susan M
AU  - Rea SM
AD  - Department of Materials Science and Metallurgy, University of Cambridge, Pembroke 
      Street, Cambridge CB2 3QZ, England. smr31@cam.ac.uk
FAU - Brooks, Roger A
AU  - Brooks RA
FAU - Schneider, Andreas
AU  - Schneider A
FAU - Best, Serena M
AU  - Best SM
FAU - Bonfield, William
AU  - Bonfield W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biomed Mater Res B Appl Biomater
JT  - Journal of biomedical materials research. Part B, Applied biomaterials
JID - 101234238
RN  - 0 (Apatites)
RN  - 0 (Bone Substitutes)
RN  - 0 (Calcium Compounds)
RN  - 0 (Silicates)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - 9002-88-4 (Polyethylene)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - S4255P4G5M (calcium silicate)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Alkaline Phosphatase/metabolism
MH  - Apatites/*pharmacology
MH  - Bone Substitutes/*pharmacology
MH  - Calcium Compounds/*pharmacology
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Humans
MH  - Microscopy, Confocal
MH  - Microscopy, Electron, Scanning
MH  - Osteoblasts/*cytology/drug effects
MH  - Polyethylene/*pharmacology
MH  - Silicates/*pharmacology
MH  - Surface Properties
EDAT- 2004/07/21 05:00
MHDA- 2005/12/13 09:00
CRDT- 2004/07/21 05:00
PHST- 2004/07/21 05:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2004/07/21 05:00 [entrez]
AID - 10.1002/jbm.b.30039 [doi]
PST - ppublish
SO  - J Biomed Mater Res B Appl Biomater. 2004 Aug 15;70(2):250-61. doi: 
      10.1002/jbm.b.30039.