PMID- 15265298
OWN - NLM
STAT- MEDLINE
DCOM- 20050104
LR  - 20210427
IS  - 1076-3279 (Print)
IS  - 1076-3279 (Linking)
VI  - 10
IP  - 5-6
DP  - 2004 May-Jun
TI  - Megakaryocyte-bone marrow stromal cell aggregates demonstrate increased colony 
      formation and alkaline phosphatase expression in vitro.
PG  - 807-17
AB  - Bone marrow stromal cells (BMSCs) possess certain stem celllike properties and 
      can differentiate to adopt a number of mesenchymal phenotypes. BMSCs are usually 
      investigated in vitro as homogeneous single-cell suspensions; however, these 
      preparations lose much of their osteogenic capacity. Using the fibroblastic 
      colony-forming unit assay, we have compared the proliferation and capacity to 
      express alkaline phosphatase of BMSC-containing aggregates of bone marrow cells 
      with single-cell suspensions of bone marrow cells from the same source. 
      Aggregates were separated from single cells by density gradient centrifugation or 
      cell sieving. The aggregate and single-cell preparations gave rise to similar 
      numbers of colonies; however, the colonies produced by the aggregates were larger 
      and expressed higher levels of alkaline phosphatase. When the aggregates were 
      dissociated, colonies still formed; however, they expressed negligible levels of 
      alkaline phosphatase. Immunomagnetic selection and immunofluorescent staining for 
      CD61, N-methyl-D-aspartate (NMDA) receptor subunit 1, and acetylcholinesterase 
      showed that the majority of the aggregates giving rise to osteoblastic colonies 
      contained megakaryocytes. These data demonstrate that removing BMSCs from their 
      normal environment reduces their osteoblastic capacity and that to achieve their 
      maximal differentiation, BMSCs require direct physical contact with accessory 
      cells such as megakaryocytes. These findings may be of direct relevance to the 
      use of BMSCs for tissue-engineering purposes.
FAU - Miao, Dengshun
AU  - Miao D
AD  - Department of Human Metabolism and Clinical Biochemistry, University of 
      Sheffield, Sheffield, UK.
FAU - Murant, Susan
AU  - Murant S
FAU - Scutt, Nanette
AU  - Scutt N
FAU - Genever, Paul
AU  - Genever P
FAU - Scutt, Andrew
AU  - Scutt A
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - Tissue Eng
JT  - Tissue engineering
JID - 9505538
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology/*physiology
MH  - Cell Aggregation/physiology
MH  - Cell Culture Techniques/*methods
MH  - Cell Differentiation/physiology
MH  - Cells, Cultured
MH  - Colony-Forming Units Assay/methods
MH  - Male
MH  - Megakaryocytes/cytology/physiology
MH  - Mesenchymal Stem Cells/*cytology/*physiology
MH  - Osteoblasts
MH  - Osteogenesis/physiology
MH  - Rats
MH  - Rats, Wistar
MH  - Stromal Cells/cytology/physiology
MH  - Tissue Engineering/*methods
EDAT- 2004/07/22 05:00
MHDA- 2005/01/05 09:00
CRDT- 2004/07/22 05:00
PHST- 2004/07/22 05:00 [pubmed]
PHST- 2005/01/05 09:00 [medline]
PHST- 2004/07/22 05:00 [entrez]
AID - 10.1089/1076327041348473 [doi]
PST - ppublish
SO  - Tissue Eng. 2004 May-Jun;10(5-6):807-17. doi: 10.1089/1076327041348473.