PMID- 15265307
OWN - NLM
STAT- MEDLINE
DCOM- 20050104
LR  - 20220408
IS  - 1076-3279 (Print)
IS  - 1076-3279 (Linking)
VI  - 10
IP  - 5-6
DP  - 2004 May-Jun
TI  - Tissue engineering of ligaments: a comparison of bone marrow stromal cells, 
      anterior cruciate ligament, and skin fibroblasts as cell source.
PG  - 893-903
AB  - Anterior cruciate ligament (ACL) reconstruction surgery still has important 
      problems to overcome, such as "donor site morbidity" and the limited choice of 
      grafts in revision surgery. Tissue engineering of ligaments may provide a 
      solution for these problems. Little is known about the optimal cell source for 
      tissue engineering of ligaments. The aim of this study is to determine the 
      optimal cell source for tissue engineering of the anterior cruciate ligament. 
      Bone marrow stromal cells (BMSCs), ACL, and skin fibroblasts were seeded onto a 
      resorbable suture material [poly(L-lactide/glycolide) multifilaments] at five 
      different seeding densities, and cultured for up to 12 days. All cell types 
      tested attached to the suture material, proliferated, and synthesized 
      extracellular matrix rich in collagen type I. On day 12 the scaffolds seeded with 
      BMSCs showed the highest DNA content (p < 0.01) and the highest collagen 
      production (p < 0.05 for the two highest seeding densities). Scaffolds seeded 
      with ACL fibroblasts showed the lowest DNA content and collagen production. 
      Accordingly, BMSCs appear to be the most suitable cells for further study and 
      development of tissue-engineered ligament.
FAU - Van Eijk, F
AU  - Van Eijk F
AD  - Department of Orthopedics, University Medical Center, Utrecht, The Netherlands.
FAU - Saris, D B F
AU  - Saris DB
FAU - Riesle, J
AU  - Riesle J
FAU - Willems, W J
AU  - Willems WJ
FAU - Van Blitterswijk, C A
AU  - Van Blitterswijk CA
FAU - Verbout, A J
AU  - Verbout AJ
FAU - Dhert, W J A
AU  - Dhert WJ
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PL  - United States
TA  - Tissue Eng
JT  - Tissue engineering
JID - 9505538
SB  - IM
MH  - Animals
MH  - Anterior Cruciate Ligament/cytology/physiology
MH  - Bone Marrow Cells/*cytology/*physiology
MH  - Cell Culture Techniques/methods
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cell Survival/physiology
MH  - Cells, Cultured
MH  - Extracellular Matrix/physiology
MH  - Fibroblasts/*cytology/*physiology
MH  - Goats
MH  - Ligaments/*cytology/*physiology
MH  - Skin/cytology
MH  - Skin Physiological Phenomena
MH  - Stromal Cells/cytology/physiology
MH  - Tissue Engineering/*methods
EDAT- 2004/07/22 05:00
MHDA- 2005/01/05 09:00
CRDT- 2004/07/22 05:00
PHST- 2004/07/22 05:00 [pubmed]
PHST- 2005/01/05 09:00 [medline]
PHST- 2004/07/22 05:00 [entrez]
AID - 10.1089/1076327041348428 [doi]
PST - ppublish
SO  - Tissue Eng. 2004 May-Jun;10(5-6):893-903. doi: 10.1089/1076327041348428.