PMID- 1526884
OWN - NLM
STAT- MEDLINE
DCOM- 19921022
LR  - 20190819
IS  - 0378-5955 (Print)
IS  - 0378-5955 (Linking)
VI  - 61
IP  - 1-2
DP  - 1992 Aug
TI  - Permeability changes of the blood-labyrinth barrier measured in vivo during 
      experimental treatments.
PG  - 12-8
AB  - The communication between blood and cochlear perilymph was investigated using the 
      tracer ion trimethylphenylammonium (TMPA). TMPA can be detected in micromolar 
      concentrations by ion-selective microelectrodes, allowing it to be used as an 
      almost ideal marker to study intercommunication between fluid compartments. 
      TMPA-sensitive electrodes were sealed into the cochlear scalae, using procedures 
      which avoided the artifactual displacement of perilymph by cerebrospinal fluid 
      (CSF). TMPA was infused intravenously at a low rate to produce a plasma 
      concentration of approximately 0.5, mM. The time course of entry into perilymph 
      of scala tympani (ST), scala vestibuli (SV) and into CSF were compared. After 90 
      min infusion, the mean CSF concentration reached 14.3% of that measured in 
      plasma. The TMPA concentrations measured in ST and SV perilymph were 
      significantly lower than those recorded in CSF, only reaching an average of 6.5% 
      and 3.7% of the plasma concentration respectively after 90 min. The slow entry of 
      TMPA confirms the existence of a tight blood-labyrinth barrier, equivalent to the 
      blood-brain or blood-CSF barriers. The rate of TMPA entry into perilymph was 
      increased by epinephrine-induced hypertension or by simultaneous administration 
      of histamine and prostaglandin E2. These treatments are presumed to increase the 
      permeability of the blood-labyrinth barrier. Characterization and manipulation of 
      blood-labyrinth barrier permeability could be important to our understanding 
      cochlear pathology.
FAU - Inamura, N
AU  - Inamura N
AD  - Department of Otolaryngology, Washington University School of Medicine, St. 
      Louis, Missouri.
FAU - Salt, A N
AU  - Salt AN
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Hear Res
JT  - Hearing research
JID - 7900445
RN  - 0 (Quaternary Ammonium Compounds)
RN  - 3426-74-2 (phenyltrimethylammonium)
RN  - 820484N8I3 (Histamine)
RN  - K7Q1JQR04M (Dinoprostone)
RN  - YKH834O4BH (Epinephrine)
SB  - IM
MH  - Animals
MH  - Cochlea/*metabolism
MH  - Dinoprostone/pharmacology
MH  - Epinephrine/pharmacology
MH  - Guinea Pigs
MH  - Histamine/pharmacology
MH  - Infusions, Intravenous
MH  - Microelectrodes
MH  - Perilymph/*metabolism
MH  - Permeability
MH  - Quaternary Ammonium Compounds/administration & dosage/blood/cerebrospinal 
      fluid/*metabolism
EDAT- 1992/08/01 00:00
MHDA- 1992/08/01 00:01
CRDT- 1992/08/01 00:00
PHST- 1992/08/01 00:00 [pubmed]
PHST- 1992/08/01 00:01 [medline]
PHST- 1992/08/01 00:00 [entrez]
AID - 0378-5955(92)90030-Q [pii]
AID - 10.1016/0378-5955(92)90030-q [doi]
PST - ppublish
SO  - Hear Res. 1992 Aug;61(1-2):12-8. doi: 10.1016/0378-5955(92)90030-q.