PMID- 15269368
OWN - NLM
STAT- MEDLINE
DCOM- 20040824
LR  - 20201208
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 85
IP  - Pt 8
DP  - 2004 Aug
TI  - Sequence analysis of human rhinoviruses in the RNA-dependent RNA polymerase 
      coding region reveals large within-species variation.
PG  - 2271-2277
LID - 10.1099/vir.0.79897-0 [doi]
AB  - Human rhinoviruses (HRVs; family Picornaviridae), the most frequent causative 
      agents of respiratory infections, comprise more than 100 distinct serotypes. 
      According to previous phylogenetic analysis of the VP4/VP2-coding sequences, all 
      but one of the HRV prototype strains distribute between the two established 
      species, Human rhinovirus A (HRV-A) and Human rhinovirus B (HRV-B). Here, partial 
      sequences of the RNA-dependent RNA polymerase (3D polymerase)-coding gene of 48 
      HRV prototype strains and 12 field isolates were analysed. The designated 
      division of the HRV strains into the species HRV-A and HRV-B was also seen in the 
      3D-coding region. Phylogenetically, HRV-B clustered closer to human enterovirus 
      (HEV) species HEV-B, HEV-C and poliovirus than to HRV-A. Intraspecies variation 
      within both HRV-A and HRV-B was greater in the 3D-coding region than in the 
      VP4/VP2-coding region, with the difference maxima reaching 48 % at the nucleotide 
      level and 36 % at the amino acid level in HRV-A and 53 and 35 %, respectively, in 
      HRV-B. Within both species, a few strains formed a separate cluster differing 
      from the majority of strains as much as HEV-B from HEV-C. Furthermore, the tree 
      topology within HRV-A differed from that for VP4/VP2, suggesting possible 
      recombination events in the evolutionary history of the strains. However, all 12 
      field isolates clustered similarly, as in the capsid region. These results showed 
      that the within-species variation in the 3D region is greater in HRV than in HEV. 
      Furthermore, HRV variation in the 3D region exceeds that in the capsid-coding 
      region.
FAU - Savolainen, Carita
AU  - Savolainen C
AD  - Enterovirus Laboratory, Department of Microbiology, National Public Health 
      Institute (KTL), Mannerheimintie 166, FIN-00300 Helsinki, Finland.
FAU - Laine, Pia
AU  - Laine P
AD  - Enterovirus Laboratory, Department of Microbiology, National Public Health 
      Institute (KTL), Mannerheimintie 166, FIN-00300 Helsinki, Finland.
FAU - Mulders, Mick N
AU  - Mulders MN
AD  - Enterovirus Laboratory, Department of Microbiology, National Public Health 
      Institute (KTL), Mannerheimintie 166, FIN-00300 Helsinki, Finland.
FAU - Hovi, Tapani
AU  - Hovi T
AD  - Enterovirus Laboratory, Department of Microbiology, National Public Health 
      Institute (KTL), Mannerheimintie 166, FIN-00300 Helsinki, Finland.
LA  - eng
SI  - GENBANK/AY436646
SI  - GENBANK/AY436647
SI  - GENBANK/AY436648
SI  - GENBANK/AY436649
SI  - GENBANK/AY436650
SI  - GENBANK/AY436651
SI  - GENBANK/AY436652
SI  - GENBANK/AY436653
SI  - GENBANK/AY436654
SI  - GENBANK/AY436655
SI  - GENBANK/AY436656
SI  - GENBANK/AY436657
SI  - GENBANK/AY436658
SI  - GENBANK/AY436659
SI  - GENBANK/AY436660
SI  - GENBANK/AY436661
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SI  - GENBANK/AY436663
SI  - GENBANK/AY436664
SI  - GENBANK/AY436665
SI  - GENBANK/AY436666
SI  - GENBANK/AY436667
SI  - GENBANK/AY436668
SI  - GENBANK/AY436669
SI  - GENBANK/AY436670
SI  - GENBANK/AY436671
SI  - GENBANK/AY436672
SI  - GENBANK/AY436673
SI  - GENBANK/AY436674
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SI  - GENBANK/AY436677
SI  - GENBANK/AY436678
SI  - GENBANK/AY436679
SI  - GENBANK/AY436680
SI  - GENBANK/AY436681
SI  - GENBANK/AY436682
SI  - GENBANK/AY436683
SI  - GENBANK/AY436684
SI  - GENBANK/AY436685
SI  - GENBANK/AY436686
SI  - GENBANK/AY436687
SI  - GENBANK/AY436688
SI  - GENBANK/AY436689
SI  - GENBANK/AY436690
SI  - GENBANK/AY436691
SI  - GENBANK/AY436692
SI  - GENBANK/AY436693
SI  - GENBANK/AY436694
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SI  - GENBANK/AY436696
SI  - GENBANK/AY436697
SI  - GENBANK/AY436698
SI  - GENBANK/AY436699
SI  - GENBANK/AY436700
SI  - GENBANK/AY436701
SI  - GENBANK/AY436702
SI  - GENBANK/AY436703
SI  - GENBANK/AY436704
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
RN  - EC 2.7.7.48 (RNA-Dependent RNA Polymerase)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Humans
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA-Dependent RNA Polymerase/chemistry/*genetics
MH  - Recombination, Genetic
MH  - Rhinovirus/classification/*genetics
MH  - Species Specificity
EDAT- 2004/07/23 05:00
MHDA- 2004/08/25 05:00
CRDT- 2004/07/23 05:00
PHST- 2004/07/23 05:00 [pubmed]
PHST- 2004/08/25 05:00 [medline]
PHST- 2004/07/23 05:00 [entrez]
AID - 10.1099/vir.0.79897-0 [doi]
PST - ppublish
SO  - J Gen Virol. 2004 Aug;85(Pt 8):2271-2277. doi: 10.1099/vir.0.79897-0.