PMID- 15272539
OWN - NLM
STAT- MEDLINE
DCOM- 20040818
LR  - 20220330
IS  - 0002-9173 (Print)
IS  - 0002-9173 (Linking)
VI  - 122
IP  - 1
DP  - 2004 Jul
TI  - Detection and quantitation of HER-2 gene amplification and protein expression in 
      breast carcinoma.
PG  - 110-9
AB  - We compared fluorescence in situ hybridization (FISH), immunohistochemical 
      analysis, immunocytochemical analysis, and relative quantification assays using 
      polymerase chain reaction (PCR) as methods for estimating HER-2 gene 
      amplification and protein overexpression infine-needle aspirate (FNA) specimens 
      and paraffin-embedded tissue samples from 49 cases of breast cancer: FISH can be 
      performed successfully on FNA smears. Immunohistochemical and immunocytochemical 
      staining intensity of 3+ corresponds to a FISH ratio of more than 2.5. 
      Immunohistochemical and immunocytochemical staining of 2+ and 1+ are not 
      necessarily associated with gene amplification. Increased DNA PCR ratios might be 
      seen without amplification, reflecting polysomy. HER-2 messenger RNA relative 
      quantitation scores correlate well with HER-2 gene amplification. Owing to the 
      ease with which it can be performed and interpreted, we conclude that FISH is the 
      test of choice for HER-2 estimation and, when possible, should be performed on 
      whole nuclei, which are readily available in FNA smears or imprint cytology. FISH 
      may be used primarily or to confirm immunohistochemical, immunocytochemical, and 
      PCR results.
FAU - Bofin, Anna M
AU  - Bofin AM
AD  - Department of Laboratory Medicine, Children's and Women's Health, Faculty of 
      Medicine, Norwegian University of Science and Technology, Trondheim.
FAU - Ytterhus, Borgny
AU  - Ytterhus B
FAU - Martin, Cara
AU  - Martin C
FAU - O'Leary, John J
AU  - O'Leary JJ
FAU - Hagmar, Bjorn M
AU  - Hagmar BM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Biomarkers, Tumor/*analysis
MH  - Biopsy, Fine-Needle
MH  - Breast Neoplasms/*genetics/*pathology
MH  - Female
MH  - *Gene Amplification
MH  - *Genes, erbB-2
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Paraffin Embedding
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2004/07/27 05:00
MHDA- 2004/08/19 05:00
CRDT- 2004/07/27 05:00
PHST- 2004/07/27 05:00 [pubmed]
PHST- 2004/08/19 05:00 [medline]
PHST- 2004/07/27 05:00 [entrez]
AID - 10.1309/8A2D-JFT0-7NE6-EWHE [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2004 Jul;122(1):110-9. doi: 10.1309/8A2D-JFT0-7NE6-EWHE.