PMID- 15273723
OWN - NLM
STAT- MEDLINE
DCOM- 20040930
LR  - 20220331
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 23
IP  - 41
DP  - 2004 Sep 9
TI  - Deregulation of the carbohydrate (chondroitin 4) sulfotransferase 11 (CHST11) 
      gene in a B-cell chronic lymphocytic leukemia with a t(12;14)(q23;q32).
PG  - 6991-6
AB  - The t(12;14)(q23;q32) breakpoints in a case of B-cell chronic lymphocytic 
      leukemia (B-CLL) were mapped by fluorescence in situ hybridization (FISH) and 
      Southern blot analysis and cloned using an IGH switch-gamma probe. The 
      translocation affected a productively rearranged IGH allele and the carbohydrate 
      (chondroitin 4) sulfotransferase 11 (CHST11) locus at 12q23, with a reciprocal 
      break in intron 2 of the CHST11 gene. CHST11 belongs to the HNK1 family of 
      Golgi-associated sulfotransferases, a group of glycosaminoglycan-modifying 
      enzymes, and is expressed mainly in the hematopoietic lineage. Northern Blot 
      analysis of tumor RNA using CHST11-specific probes showed expression of two 
      CHST11 forms of abnormal size. 5'- and 3'-Rapid Amplification of cDNA Ends (RACE) 
      revealed IGH/CHST11 as well as CHST11/IGH fusion RNAs expressed from the der(14) 
      and der(12) chromosomes. Both fusion species contained open reading frames making 
      possible the translation of two truncated forms of CHST11 protein. The biological 
      consequence of t(12;14)(q23;q32) in this case presumably is a disturbance of the 
      cellular distribution of CHST11 leading to deregulation of a 
      chondroitin-sulfate-dependent pathway specific to the hematopoietic lineage.
FAU - Schmidt, Helmut H
AU  - Schmidt HH
AD  - Cell Biology Program, and Department of Medicine, Memorial Sloan-Kettering Cancer 
      Center, New York, NY, USA. helmut.schmidt@klinikum-graz.at
FAU - Dyomin, Vadim G
AU  - Dyomin VG
FAU - Palanisamy, Nallasivam
AU  - Palanisamy N
FAU - Itoyama, Takahiro
AU  - Itoyama T
FAU - Nanjangud, Gouri
AU  - Nanjangud G
FAU - Pirc-Danoewinata, Hendrati
AU  - Pirc-Danoewinata H
FAU - Haas, Oskar A
AU  - Haas OA
FAU - Chaganti, R S K
AU  - Chaganti RS
LA  - eng
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - EC 2.8.2- (CHST11 protein, human)
RN  - EC 2.8.2.- (Sulfotransferases)
SB  - IM
MH  - Base Sequence
MH  - *Chromosomes, Human, Pair 12
MH  - *Chromosomes, Human, Pair 14
MH  - *Gene Expression Regulation, Leukemic
MH  - Humans
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Sulfotransferases/*genetics
MH  - *Translocation, Genetic
EDAT- 2004/07/27 05:00
MHDA- 2004/10/02 05:00
CRDT- 2004/07/27 05:00
PHST- 2004/07/27 05:00 [pubmed]
PHST- 2004/10/02 05:00 [medline]
PHST- 2004/07/27 05:00 [entrez]
AID - 1207934 [pii]
AID - 10.1038/sj.onc.1207934 [doi]
PST - ppublish
SO  - Oncogene. 2004 Sep 9;23(41):6991-6. doi: 10.1038/sj.onc.1207934.