PMID- 15274157
OWN - NLM
STAT- MEDLINE
DCOM- 20080207
LR  - 20040726
IS  - 1522-726X (Electronic)
IS  - 1522-1946 (Linking)
VI  - 62
IP  - 4
DP  - 2004 Aug
TI  - Effects of contrast media on porcine bone marrow-derived mononuclear cells and 
      calf myoblast viability and secretion of VEGF and MCP-1.
PG  - 476-81
AB  - We investigated the effect of contrast media on bone marrow-derived cell 
      viability, growth factor secretion, and myoblast viability. Bone marrow was 
      exposed to contrast media, mononuclear cells were isolated, viability was 
      assessed by Trypan blue exclusion or cultured for 4 weeks, and conditioned medium 
      was assayed for vascular endothelial growth factor (VEGF) and monocyte 
      chemoattractant protein-1 (MCP-1). Skeletal myoblasts viability was assessed 
      after exposing them to contrast media. In separate experiments, bone marrow or 
      bone marrow-derived mononuclear cells were exposed to contrast media, cultured 
      for 40 hr, then assessed for viability. None of the contrast media tested had any 
      effect on bone marrow-derived cell viability. Hypaque or Hexabrix increased 
      myoblasts viability by 8-10%. VEGF and MCP-1 concentrations in the conditioned 
      medium increased in a time-related manner. These findings support the concept 
      that for cell therapy, bone marrow cells or myoblasts may be mixed with contrast 
      media and injected into ischemic myocardium without compromise in viability or 
      function.
CI  - Copyright 2004 Wiley-Liss, Inc.
FAU - Baffour, Richard
AU  - Baffour R
AD  - Cardiovascular Research Institute, Washington Hospital Center, Washington, 
      District of Columbia.
FAU - Pakala, Rajbabu
AU  - Pakala R
FAU - Hellinga, David
AU  - Hellinga D
FAU - Seabron, Rufus
AU  - Seabron R
FAU - Fournadjiev, Jana
AU  - Fournadjiev J
FAU - Wolfram, Roswitha
AU  - Wolfram R
FAU - Okubagzi, Petros
AU  - Okubagzi P
FAU - Epstein, Stephen E
AU  - Epstein SE
FAU - Waksman, Ron
AU  - Waksman R
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Catheter Cardiovasc Interv
JT  - Catheterization and cardiovascular interventions : official journal of the 
      Society for Cardiac Angiography & Interventions
JID - 100884139
RN  - 0 (Chemokine CCL2)
RN  - 0 (Contrast Media)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*drug effects/metabolism
MH  - Cattle
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL2/*metabolism
MH  - Contrast Media/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Myoblasts, Skeletal/*drug effects
MH  - Swine
MH  - Time Factors
MH  - Vascular Endothelial Growth Factor A/*metabolism
EDAT- 2004/07/27 05:00
MHDA- 2008/02/08 09:00
CRDT- 2004/07/27 05:00
PHST- 2004/07/27 05:00 [pubmed]
PHST- 2008/02/08 09:00 [medline]
PHST- 2004/07/27 05:00 [entrez]
AID - 10.1002/ccd.20096 [doi]
PST - ppublish
SO  - Catheter Cardiovasc Interv. 2004 Aug;62(4):476-81. doi: 10.1002/ccd.20096.