PMID- 15274652
OWN - NLM
STAT- MEDLINE
DCOM- 20050210
LR  - 20181130
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 52
IP  - 2
DP  - 2004 Aug
TI  - The effect of inflammatory cytokines on secretion of macrophage 
      colony-stimulating factor and monocyte chemoattractant protein-1 in human 
      granulosa cells.
PG  - 124-8
AB  - PROBLEM: In order to investigate the role of macrophage colony-stimulating factor 
      (M-CSF) and monocyte chemoattractant protein -1 (MCP-1) in human ovulation, we 
      studied the regulation of M-CSF and MCP-1 in cultured human granulosa cells. 
      METHOD OF STUDY: Immortalized granulosa cells (GC1a) were cultured in serum-free 
      medium, and incubated with interleukin (IL)-1alpha, IL-1 receptor antagonist (ra) 
      and tumor necrosis factor (TNF)-alpha. The supernatants were collected, and M-CSF 
      and MCP-1 were measured by ELISA. RESULTS: The levels of M-CSF and MCP-1 were 
      increased after treatment with IL-1alpha (1 nm) and TNF-alpha (1 nm) in a 
      time-dependent manner. The levels of M-CSF and MCP-1 were significantly increased 
      after treatment with IL-1alpha and TNF-alpha in a dose-dependent manner. However, 
      the levels of M-CSF and MCP-1 were significantly decreased by treatment with 
      IL-1alpha (1 nm) and/or increasing concentrations of IL-1 ra. CONCLUSIONS: Our 
      data indicated that M-CSF and MCP-1 were regulated by IL-1alpha and TNF-alpha. It 
      was suggested that M-CSF and MCP-1 may play an important role in human 
      preovulatory processes.
FAU - Kawano, Yasushi
AU  - Kawano Y
AD  - Department of Obstetrics and Gynecology, Faculty of Medicine, Oita University, 
      Oita, Japan. kawayas@med.oita-u.ac.jp
FAU - Fukuda, Junichiro
AU  - Fukuda J
FAU - Itoh, Hiroko
AU  - Itoh H
FAU - Takai, Noriyuki
AU  - Takai N
FAU - Nasu, Kaei
AU  - Nasu K
FAU - Miyakawa, Isao
AU  - Miyakawa I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (Chemokine CCL2)
RN  - 0 (Culture Media, Serum-Free)
RN  - 0 (Interleukin-1)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Cell Line, Transformed
MH  - Chemokine CCL2/*metabolism
MH  - Culture Media, Serum-Free
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Granulosa Cells/*metabolism
MH  - Humans
MH  - Interleukin-1/*metabolism/pharmacology
MH  - Macrophage Colony-Stimulating Factor/*metabolism
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/*metabolism/pharmacology
EDAT- 2004/07/28 05:00
MHDA- 2005/02/11 09:00
CRDT- 2004/07/28 05:00
PHST- 2004/07/28 05:00 [pubmed]
PHST- 2005/02/11 09:00 [medline]
PHST- 2004/07/28 05:00 [entrez]
AID - AJI198 [pii]
AID - 10.1111/j.1600-0897.2004.00198.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 2004 Aug;52(2):124-8. doi: 10.1111/j.1600-0897.2004.00198.x.