PMID- 1527523
OWN - NLM
STAT- MEDLINE
DCOM- 19921016
LR  - 20151119
IS  - 0022-3034 (Print)
IS  - 0022-3034 (Linking)
VI  - 23
IP  - 2
DP  - 1992 Mar
TI  - Glycoconjugates are stage- and position-specific cell surface molecules in the 
      developing olfactory system, 2: Unique carbohydrate antigens are topographic 
      markers for selective projection patterns of olfactory axons.
PG  - 130-42
AB  - Three monoclonal antibodies specific for different carbohydrate antigens were 
      used to analyze the development of the olfactory system in rats. CC2 antibodies 
      react with a subset of main olfactory neurons, their axons, and terminals in the 
      olfactory bulb. CC2 antigens are expressed on dorsomedial neurons in the 
      olfactory epithelium (OE) from embryonic (E) day 15 to adults. In the olfactory 
      bulb (OB), only dorsomedially located glomeruli express CC2 glycoconjugates from 
      postnatal day (P) 2 to adults. Thus CC2 defines a dorsomedially organized 
      projection that is established early in embryonic development and continues in 
      adults. P-Path antibodies react with antigens that are expressed on the olfactory 
      nerve in embryos, and are also detected on cell bodies in the neuroepithelium and 
      in glomeruli of the OB at P2. At P14, P-Path staining is weaker, but remains 
      present on many cells in the epithelium and in many glomeruli in the bulb. 
      Postnatally, P-Path immunostaining continues to decrease in most regions of the 
      OE and OB. At P35 and afterwards, only a few P-Path-positive neuronal cells can 
      be detected in the OE. Furthermore, after P35 only two groups of glomeruli in the 
      OB are P-Path immunoreactive. One is situated adjacent to the accessory olfactory 
      bulb (AOB) at the dorsocaudal surface of the OB. The other is adjacent to the AOB 
      at the ventrocaudal surface of the OB. Thus, in adults, P-Path glycoconjugates 
      are expressed in neurons and axons that project only to a specific subset of 
      caudal glomeruli of the OB. Monoclonal antibody 1B2, reacts with 
      beta-galactose-terminating glycolipids and glycoproteins. At P2, 1B2 
      immunoreactivity is seen on a subset of cell bodies that are distributed 
      throughout the OE and is expressed in most glomeruli in the OB at this age. By 
      P35 and in adults, 1B2 continues to be expressed on a subset of neurons in the OE 
      that project to only a small subset of glomeruli in the OB. Unlike CC2 and P-Path 
      antigens that define specific groups of glomeruli, 1B2-immunoreactive glomeruli 
      do not have a detectable spatial pattern. It is more likely that 1B2 antigens 
      define a specific stage in the maturation of connections between the OE and OB.
FAU - Schwarting, G A
AU  - Schwarting GA
AD  - Department of Biochemistry, E. K. Shriver Center, Waltham, Massachusetts 02254.
FAU - Deutsch, G
AU  - Deutsch G
FAU - Gattey, D M
AU  - Gattey DM
FAU - Crandall, J E
AU  - Crandall JE
LA  - eng
GR  - HD05515/HD/NICHD NIH HHS/United States
GR  - NS 24386/NS/NINDS NIH HHS/United States
GR  - NS 25580/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurobiol
JT  - Journal of neurobiology
JID - 0213640
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigens, Surface)
RN  - 0 (Biomarkers)
RN  - 0 (Carbohydrates)
RN  - 0 (Glycoconjugates)
RN  - 0 (Membrane Lipids)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - Antigens, Surface/*analysis
MH  - Axons/*physiology
MH  - Biomarkers/chemistry
MH  - Brain Mapping/methods
MH  - Carbohydrates/*immunology
MH  - Cells, Cultured
MH  - Glycoconjugates/*analysis
MH  - Membrane Lipids/*analysis
MH  - Olfactory Bulb/*chemistry/embryology/growth & development
MH  - Rats
MH  - Rats, Inbred Strains
EDAT- 1992/03/01 00:00
MHDA- 1992/03/01 00:01
CRDT- 1992/03/01 00:00
PHST- 1992/03/01 00:00 [pubmed]
PHST- 1992/03/01 00:01 [medline]
PHST- 1992/03/01 00:00 [entrez]
AID - 10.1002/neu.480230204 [doi]
PST - ppublish
SO  - J Neurobiol. 1992 Mar;23(2):130-42. doi: 10.1002/neu.480230204.