PMID- 15276362
OWN - NLM
STAT- MEDLINE
DCOM- 20050321
LR  - 20201208
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 26
IP  - 5
DP  - 2005 Feb
TI  - Effect of functionalization of multilayered polyelectrolyte films on motoneuron 
      growth.
PG  - 545-54
AB  - We studied in vitro cell-substrate interaction of motoneurons with functionalized 
      polylectrolyte films. Thin polylectrolyte films were built on glass by 
      alternating polycations, poly(ethylene-imine) PEI, poly(L-lysine) PLL, or 
      poly(allylamine hydrochloride) PAH, and polyanions, 
      poly(sodium-4-styrenesulfonate) PSS or poly(L-glutamic acid) (PGA). These 
      architectures were functionalized with Brain Derived Neurotrophic Factor (BDNF) 
      or Semaphorin 3A (Sema3A). We used Optical Waveguide Lightmode Spectroscopy 
      (OWLS) and Atomic Force Microscopy (AFM) to characterize the architectures. The 
      viability of motoneurons was estimated by the acid phosphatase method, and 
      morphometrical measures were performed to analyse the influence of different 
      architectures on cell morphology. Motoneurons appeared to adhere and spread on 
      all the architectures tested and preferentially on PSS ending films. The 
      viability of motoneurons on polyelectrolyte multilayers was higher compared to 
      polyelectrolyte monolayers. BDNF and Sema3A embedded in the films remained active 
      and thereby create functionalized nanofilms.
FAU - Vodouhe, Constant
AU  - Vodouhe C
AD  - INSERM Unite 595, 11 rue Humann, Strasbourg Cedex 67085, France.
FAU - Schmittbuhl, Matthieu
AU  - Schmittbuhl M
FAU - Boulmedais, Fouzia
AU  - Boulmedais F
FAU - Bagnard, Dominique
AU  - Bagnard D
FAU - Vautier, Dominique
AU  - Vautier D
FAU - Schaaf, Pierre
AU  - Schaaf P
FAU - Egles, Christophe
AU  - Egles C
FAU - Voegel, Jean-Claude
AU  - Voegel JC
FAU - Ogier, Joelle
AU  - Ogier J
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Anions)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (DNA, Complementary)
RN  - 0 (Electrolytes)
RN  - 0 (Polyamines)
RN  - 0 (Polyelectrolytes)
RN  - 0 (Polymers)
RN  - 0 (Semaphorin-3A)
RN  - 0 (Sulfonic Acids)
RN  - 0 (polycations)
RN  - 25104-18-1 (Polylysine)
RN  - 25513-46-6 (Polyglutamic Acid)
RN  - 30551-89-4 (polyallylamine)
RN  - 9002-98-6 (Polyethyleneimine)
RN  - ZSL2FB6GXN (styrenesulfonic acid polymer)
SB  - IM
MH  - Animals
MH  - Anions/*pharmacology
MH  - Brain-Derived Neurotrophic Factor/administration & dosage/*pharmacology
MH  - Cell Adhesion/drug effects
MH  - Cell Culture Techniques/*instrumentation
MH  - Cells, Cultured
MH  - DNA, Complementary/genetics
MH  - Electrolytes/*pharmacology
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - Kidney
MH  - Materials Testing
MH  - Mice
MH  - Microscopy, Atomic Force
MH  - Motor Neurons/cytology/*drug effects
MH  - *Nanostructures
MH  - Neurites/ultrastructure
MH  - Polyamines/*pharmacology
MH  - Polyelectrolytes
MH  - Polyethyleneimine/pharmacology
MH  - Polyglutamic Acid/pharmacology
MH  - Polylysine/pharmacology
MH  - Polymers/pharmacology
MH  - Semaphorin-3A/administration & dosage/genetics/*pharmacology
MH  - Spectrum Analysis/methods
MH  - Spinal Cord/cytology
MH  - Sulfonic Acids/pharmacology
MH  - Tissue Engineering/*instrumentation
MH  - Transfection
EDAT- 2004/07/28 05:00
MHDA- 2005/03/22 09:00
CRDT- 2004/07/28 05:00
PHST- 2004/01/27 00:00 [received]
PHST- 2004/02/16 00:00 [accepted]
PHST- 2004/07/28 05:00 [pubmed]
PHST- 2005/03/22 09:00 [medline]
PHST- 2004/07/28 05:00 [entrez]
AID - S0142961204002066 [pii]
AID - 10.1016/j.biomaterials.2004.02.057 [doi]
PST - ppublish
SO  - Biomaterials. 2005 Feb;26(5):545-54. doi: 10.1016/j.biomaterials.2004.02.057.