PMID- 15279665
OWN - NLM
STAT- MEDLINE
DCOM- 20050201
LR  - 20191210
IS  - 0141-9854 (Print)
IS  - 0141-9854 (Linking)
VI  - 26
IP  - 4
DP  - 2004 Aug
TI  - Evaluation of refrigerated platelet concentrates supplemented with low doses of 
      second messenger effectors.
PG  - 275-86
AB  - With the goal of producing haemostatically effective platelet concentrates (PCs) 
      with a longer shelf-life, we aimed to identify a simple combination of platelet 
      inhibitors, with a low pharmacological load, which could avoid the unacceptable 
      loss of platelets stored under refrigerated conditions. PCs stored with different 
      combinations of second messenger effectors were analysed at days 5, 10 and 15 of 
      storage and compared with those supplemented with ThromboSol--a combination of 
      six platelet inhibitors that protects cells from cold damage. The following 
      parameters were analysed: platelet counts, biochemical parameters (glucose, pH, 
      bicarbonate, lactate), cell lysis (lactic dehydrogenase, LDH), membrane 
      glycoproteins (GPs), platelet aggregation, fibrinogen binding and hypotonic shock 
      response. We characterized the combination of amiloride and sodium nitroprusside 
      (at 1/2 the dose included in ThromboSol). This was found to be similar to 
      ThromboSol and superior to nontreated units in the prevention of cold-induced 
      platelet aggregation at day 15 of storage (maintenance of 78% and 80% of initial 
      platelet counts, respectively), preservation of GPIbalpha (11% and 12% better 
      maintenance of mean fluorescence intensity compared with control units, 
      respectively), and reduced cell lysis (13% and 11% decrease in supernatant LDH, 
      respectively). The reduced pharmacological load with the identified solution 
      compared with ThromboSol is an argument in favour of the potential use of these 
      agents when designing strategies to improve PC storage.
FAU - Perez-Ceballos, E
AU  - Perez-Ceballos E
AD  - Centro Regional de Hemodonacion and Unit of Hematology and Clinical Oncology of 
      the Hospital Morales Meseguer, Murcia, Spain.
FAU - Rivera, J
AU  - Rivera J
FAU - Lozano, M L
AU  - Lozano ML
FAU - Candela, M J
AU  - Candela MJ
FAU - Corral, J
AU  - Corral J
FAU - Guerrero, J A
AU  - Guerrero JA
FAU - Vicente, V
AU  - Vicente V
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Lab Haematol
JT  - Clinical and laboratory haematology
JID - 7907061
RN  - 0 (Cryoprotective Agents)
SB  - IM
MH  - Blood Platelets/*drug effects/physiology
MH  - Blood Preservation/*methods
MH  - Cryoprotective Agents/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Flow Cytometry
MH  - Humans
MH  - Platelet Aggregation/drug effects
MH  - Platelet Count
MH  - Refrigeration
MH  - *Second Messenger Systems
MH  - Temperature
EDAT- 2004/07/29 05:00
MHDA- 2005/02/03 09:00
CRDT- 2004/07/29 05:00
PHST- 2004/07/29 05:00 [pubmed]
PHST- 2005/02/03 09:00 [medline]
PHST- 2004/07/29 05:00 [entrez]
AID - CLH619 [pii]
AID - 10.1111/j.1365-2257.2004.00619.x [doi]
PST - ppublish
SO  - Clin Lab Haematol. 2004 Aug;26(4):275-86. doi: 10.1111/j.1365-2257.2004.00619.x.