PMID- 15279703
OWN - NLM
STAT- MEDLINE
DCOM- 20040913
LR  - 20181130
IS  - 0882-8245 (Print)
IS  - 0882-8245 (Linking)
VI  - 17
IP  - 2
DP  - 2004
TI  - Modulation of dengue virus infection of dendritic cells by Aedes aegypti saliva.
PG  - 252-65
AB  - Dengue virus (DV) is a flavivirus carried by the Aedes aegypti mosquito that 
      causes a spectrum of illnesses in the tropics, including dengue fever, dengue 
      hemorrhagic fever, and dengue shock syndrome. Dendritic cells (DCs) are 
      professional antigen presenting cells recently shown to be permissive for DV, and 
      implicated as the primary targets of initial DV infection. DV is transmitted to 
      human host by infected mosquitoes during a blood meal, but it is currently 
      unknown whether transmission is modified by vector saliva that is also deposited 
      in the host's skin during feeding. Previous studies evaluated only the outcome of 
      DV infection of DCs, and did not address the influence of mosquito saliva. To 
      more fully characterize natural transmission of DV, we evaluated the effects of 
      Ae. aegypti saliva on DV infection of human myeloid DCs. We found that saliva 
      inhibited DV infection in DCs. Moreover, pre-sensitization of DCs with saliva, 
      prior to DV infection, enhanced inhibition. In addition, enhanced production of 
      IL-12p70 and TNF-alpha were detected in DV-infected DC cultures exposed to 
      mosquito saliva. The proportion of dead cells was also significantly reduced in 
      these cultures. These data contribute to the overall understanding of the natural 
      pathogenesis of DV infection and suggest that there is a protective role for 
      mosquito saliva that limits viral uptake by DCs.
CI  - Copyright Mary Ann Liebert, Inc.
FAU - Ader, Daniel B
AU  - Ader DB
AD  - Department of Virus Diseases, Walter Reed Army Institute of Research, Silver 
      Spring, Maryland 20810, USA.
FAU - Celluzzi, Christina
AU  - Celluzzi C
FAU - Bisbing, John
AU  - Bisbing J
FAU - Gilmore, Lara
AU  - Gilmore L
FAU - Gunther, Vicky
AU  - Gunther V
FAU - Peachman, Kristina K
AU  - Peachman KK
FAU - Rao, Mangala
AU  - Rao M
FAU - Barvir, Dave
AU  - Barvir D
FAU - Sun, Wellington
AU  - Sun W
FAU - Palmer, Dupeh R
AU  - Palmer DR
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Viral Immunol
JT  - Viral immunology
JID - 8801552
RN  - 0 (Cytokines)
SB  - IM
MH  - Aedes/*cytology
MH  - Animals
MH  - Cytokines/*biosynthesis/metabolism
MH  - Dendritic Cells/pathology/*virology
MH  - Dengue Virus/*physiology
MH  - Saliva/immunology/*physiology/virology
EDAT- 2004/07/29 05:00
MHDA- 2004/09/14 05:00
CRDT- 2004/07/29 05:00
PHST- 2004/07/29 05:00 [pubmed]
PHST- 2004/09/14 05:00 [medline]
PHST- 2004/07/29 05:00 [entrez]
AID - 10.1089/0882824041310496 [doi]
PST - ppublish
SO  - Viral Immunol. 2004;17(2):252-65. doi: 10.1089/0882824041310496.