PMID- 15279836
OWN - NLM
STAT- MEDLINE
DCOM- 20040916
LR  - 20071114
IS  - 0027-5107 (Print)
IS  - 0027-5107 (Linking)
VI  - 562
IP  - 1-2
DP  - 2004 Aug 8
TI  - Inter-rater agreement on chromosome 5 breakage in FISH-based mutagen sensitivity 
      assays (MSAs).
PG  - 133-42
AB  - In chromosome breakage assays, validated, universal criteria for selection of 
      cells and classification of chromosome aberrations may enhance their utility for 
      cancer susceptibility screening. To standardize a fluorescence in situ 
      hybridization (FISH) modification of the mutagen sensitivity assay (MSA), scoring 
      criteria were evaluated by web-based validation. Two hundred digital FISH images 
      were assigned random identification numbers. With this set of images, criteria 
      for inclusion of cells and measurement of the frequency of abnormal cells were 
      evaluated by eight observers, all of whom had five or more years of experience. 
      Observers included doctoral and MS/BS level cytogeneticists, and were drawn from 
      a randomized pool of 54 volunteers. Questions addressed were: (1) how uniformly 
      were criteria applied to analysis of a standard digital FISH image set and (2) 
      did concordance vary with educational level? These data suggest inter-rater 
      agreement within a factor of 2 for average breakage frequency, but revealed 
      greater variability in cell selection. These results aid in estimating the 
      components of assay variance due to definitions, technical parameters and 
      biological variables.
FAU - Barker, Peter E
AU  - Barker PE
AD  - DNA Technologies Group, Biotechnology Division, Chemical Sciences and Technology 
      Laboratory, NIST-NCI Biomarkers Validation Project, National Institute of 
      Standards and Technology (NIST), Gaithersburg, MD 20899-8311, USA. 
      peter.barker@nist.gov
FAU - Wang, Wendy
AU  - Wang W
FAU - Wagner, Paul D
AU  - Wagner PD
FAU - Pinsky, Paul
AU  - Pinsky P
LA  - eng
GR  - Y1-CN-0103-01/CN/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Netherlands
TA  - Mutat Res
JT  - Mutation research
JID - 0400763
RN  - 0 (Mutagens)
SB  - IM
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 5
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Mutagens/*toxicity
MH  - *Observer Variation
EDAT- 2004/07/29 05:00
MHDA- 2004/09/17 05:00
CRDT- 2004/07/29 05:00
PHST- 2004/02/20 00:00 [received]
PHST- 2004/05/13 00:00 [revised]
PHST- 2004/06/03 00:00 [accepted]
PHST- 2004/07/29 05:00 [pubmed]
PHST- 2004/09/17 05:00 [medline]
PHST- 2004/07/29 05:00 [entrez]
AID - S1383571804001354 [pii]
AID - 10.1016/j.mrgentox.2004.06.001 [doi]
PST - ppublish
SO  - Mutat Res. 2004 Aug 8;562(1-2):133-42. doi: 10.1016/j.mrgentox.2004.06.001.