PMID- 15279945
OWN - NLM
STAT- MEDLINE
DCOM- 20040923
LR  - 20131121
IS  - 0167-7012 (Print)
IS  - 0167-7012 (Linking)
VI  - 58
IP  - 3
DP  - 2004 Sep
TI  - Development of multi-color FISH method for analysis of seven Bifidobacterium 
      species in human feces.
PG  - 413-21
AB  - We have developed a multi-color fluorescence in situ hybridization (FISH) method 
      which detects, by a single reaction, all seven species of Bifidobacterium (B. 
      adolescentis, B. angulatum, B. bifidum, B. breve, B. catenulatum, B. dentium, and 
      B. longum), the dominant bacteria in human feces. First, eight new types of 
      oligonucleotide probe were designed, complementary with the 16S rRNA sequence 
      specific to genus Bifidobacterium and each bifidobacterial species described 
      above. Using whole cell hybridization, the fluorescent intensity was measured 
      against the bacterial species targeted by each probe, to show that each probe is 
      specific to the targeted bacteria and that the relative fluorescent intensity 
      (RFI) as an indicator of probe accessibility is high at 61-117%. Then, bacterial 
      species-specific probes were labeled with fluorochromes (FITC, TAMRA and Cy5) in 
      seven different ways, singly or in combination. Using these probes, seven species 
      of Bifidobacterium were differentially stained in mixed samples of cultured 
      bacteria and feces from adult volunteers, proving the efficacy of this technique.
FAU - Takada, Toshihiko
AU  - Takada T
AD  - Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, 
      Tokyo 186-8650, Japan.
FAU - Matsumoto, Kazumasa
AU  - Matsumoto K
FAU - Nomoto, Koji
AU  - Nomoto K
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Microbiol Methods
JT  - Journal of microbiological methods
JID - 8306883
RN  - 0 (5-carboxytetramethylrhodamine succinimidyl ester)
RN  - 0 (Carbocyanines)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Rhodamines)
RN  - 0 (cyanine dye 5)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
MH  - Bifidobacterium/genetics/*isolation & purification
MH  - Carbocyanines/chemistry
MH  - DNA, Bacterial/chemistry/genetics
MH  - Feces/*microbiology
MH  - Fluorescein-5-isothiocyanate/chemistry
MH  - Fluorescent Dyes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Microscopy, Fluorescence
MH  - RNA, Ribosomal, 16S/chemistry/genetics
MH  - Rhodamines/chemistry
MH  - Sensitivity and Specificity
EDAT- 2004/07/29 05:00
MHDA- 2004/09/24 05:00
CRDT- 2004/07/29 05:00
PHST- 2004/02/23 00:00 [received]
PHST- 2004/05/14 00:00 [revised]
PHST- 2004/05/14 00:00 [accepted]
PHST- 2004/07/29 05:00 [pubmed]
PHST- 2004/09/24 05:00 [medline]
PHST- 2004/07/29 05:00 [entrez]
AID - S0167701204001435 [pii]
AID - 10.1016/j.mimet.2004.05.006 [doi]
PST - ppublish
SO  - J Microbiol Methods. 2004 Sep;58(3):413-21. doi: 10.1016/j.mimet.2004.05.006.