PMID- 15280036
OWN - NLM
STAT- MEDLINE
DCOM- 20040903
LR  - 20071115
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 571
IP  - 1-3
DP  - 2004 Jul 30
TI  - Specificity determinants for chemokine recognition identified using eotaxin-MCP-1 
      chimeras.
PG  - 166-70
AB  - To identify the elements of two chemokines [monocyte chemoattractant protein-1 
      (MCP-1) and eotaxin] that control their differential recognition by their 
      respective receptors (CCR2 and CCR3), we have studied the receptor interactions 
      of MCP-1-eotaxin chimeras. Each receptor was found to exhibit a distinct binding 
      preference for proteins containing the amino-terminal region of the cognate 
      chemokine for that receptor. However, other elements dictating chemokine 
      preference were different for the two receptors. In some cases, the influence of 
      replacing a particular region was dependent on the identities of neighboring 
      regions, indicating a complex network of cooperative and/or compensating 
      interactions.
FAU - Mayer, Michael R
AU  - Mayer MR
AD  - Department of Chemistry, Indiana University, Bloomington, IN 47405-0001, USA.
FAU - Parody, Todd R
AU  - Parody TR
FAU - Datta-Mannan, Amita
AU  - Datta-Mannan A
FAU - Stone, Martin J
AU  - Stone MJ
LA  - eng
GR  - GM 55055/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Chemokine CCL11)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
RN  - 0 (Chemokines, CC)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Recombinant Fusion Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Binding Sites
MH  - Chemokine CCL11
MH  - Chemokine CCL2/chemistry/genetics/*metabolism
MH  - Chemokines/pharmacology
MH  - Chemokines, CC/chemistry/genetics/*metabolism
MH  - Cloning, Molecular
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction
MH  - Protein Conformation
MH  - Receptors, Chemokine/drug effects/physiology
MH  - Recombinant Fusion Proteins/chemistry/metabolism
MH  - Sensitivity and Specificity
EDAT- 2004/07/29 05:00
MHDA- 2004/09/04 05:00
CRDT- 2004/07/29 05:00
PHST- 2004/05/13 00:00 [received]
PHST- 2004/06/09 00:00 [revised]
PHST- 2004/06/14 00:00 [accepted]
PHST- 2004/07/29 05:00 [pubmed]
PHST- 2004/09/04 05:00 [medline]
PHST- 2004/07/29 05:00 [entrez]
AID - S0014579304008373 [pii]
AID - 10.1016/j.febslet.2004.06.079 [doi]
PST - ppublish
SO  - FEBS Lett. 2004 Jul 30;571(1-3):166-70. doi: 10.1016/j.febslet.2004.06.079.