PMID- 15280805
OWN - NLM
STAT- MEDLINE
DCOM- 20040909
LR  - 20190919
IS  - 1665-2681 (Print)
IS  - 1665-2681 (Linking)
VI  - 1
IP  - 4
DP  - 2002 Oct-Dec
TI  - Effect of S-nitrosoglutathione (GSNO) added to the University of Wisconsin 
      solution (UW): II) Functional response to cold preservation/reperfusion of rat 
      liver.
PG  - 183-91
AB  - Livers cold preserved in University of Wisconsin (UW) solution followed by 
      reperfusion suffer ischemia/reperfusion injuries. Microcirculation is the primary 
      target of damage, characterized by sinusoidal perfusion failure due, mainly, to 
      morphological changes of sinusoidal endothelial cells. Here, we demonstrated that 
      the addition of S-nitrosoglutathione (GSNO) to the UW solution before cold 
      storage, as a nitric oxide (NO) donor, attenuated hepatic injuries.Wistar adult 
      rat livers were stored in UW solution (0 degrees C-48 hs) and then reperfused 
      during 60 minutes using the Isolated Perfused Rat Model (IPRL). We assayed four 
      GSNO concentration (50, 100, 250 and 500 mM). NO concentration was estimated 
      calculating the amount of nitrite (NO2-) generated in the UW solution. Injuries 
      during cold preservation were established measuring lactate dehydrogenase (LDH) 
      released to the UW solution. Meanwhile, intrahepatic resistance (IR), LDH 
      released to the perfusate, the effluent/perfusate ratio for K+, bile flow, liver 
      glycogen content and sinusoidal endothelial cell morphology were studied after 1 
      hour of reperfusion in the IPRL system. In cold preserved livers without GSNO, 
      glycogen content was dramatically reduced, IR increased markedly, LDH released 
      was high, bile flow diminished and sinusoidal endothelial cells appeared rounded 
      and detached from perisinusoidal matrix after reperfusion. The presence of 100 mM 
      GSNO prevented the IR rise and LDH release, improved bile production and 
      partially reduced endothelial cells damages. In conclusion, the addition of 100 
      mM GSNO to UW solution improved hemodynamic and function capacity of cold 
      preserved/reperfused livers.
FAU - Quintana, Alejandra B
AU  - Quintana AB
AD  - Morfologia, Departamento de Ciencias Biologicas, Fundacao Oswaldo Cruz (FIOCURZ), 
      Rio de Janeiro, Brasil.
FAU - Lenzi, Henrique L
AU  - Lenzi HL
FAU - Almada, Luciana L
AU  - Almada LL
FAU - Scandizzi, Angel L
AU  - Scandizzi AL
FAU - Furno, Graciela
AU  - Furno G
FAU - Rodriguez, Joaquin V
AU  - Rodriguez JV
FAU - Guibert, Edgardo E
AU  - Guibert EE
LA  - eng
PT  - Journal Article
PL  - Mexico
TA  - Ann Hepatol
JT  - Annals of hepatology
JID - 101155885
RN  - 0 (Insulin)
RN  - 0 (Nitric Oxide Donors)
RN  - 0 (Organ Preservation Solutions)
RN  - 0 (University of Wisconsin-lactobionate solution)
RN  - 57564-91-7 (S-Nitrosoglutathione)
RN  - 63CZ7GJN5I (Allopurinol)
RN  - GAN16C9B8O (Glutathione)
RN  - K72T3FS567 (Adenosine)
RN  - N5O3QU595M (Raffinose)
SB  - IM
MH  - *Adenosine
MH  - *Allopurinol
MH  - Animals
MH  - Cryopreservation/*methods
MH  - *Glutathione
MH  - *Insulin
MH  - Liver/*drug effects/pathology/physiopathology
MH  - Male
MH  - Nitric Oxide Donors/*pharmacology
MH  - *Organ Preservation Solutions
MH  - *Raffinose
MH  - Rats
MH  - Rats, Wistar
MH  - Reperfusion/*methods
MH  - S-Nitrosoglutathione/*pharmacology
EDAT- 2004/07/29 05:00
MHDA- 2004/09/10 05:00
CRDT- 2004/07/29 05:00
PHST- 2002/10/01 00:00 [received]
PHST- 2004/07/29 05:00 [pubmed]
PHST- 2004/09/10 05:00 [medline]
PHST- 2004/07/29 05:00 [entrez]
AID - S1665-2681(19)32164-7 [pii]
PST - ppublish
SO  - Ann Hepatol. 2002 Oct-Dec;1(4):183-91.