PMID- 15281913
OWN - NLM
STAT- MEDLINE
DCOM- 20050511
LR  - 20191210
IS  - 1470-8728 (Electronic)
IS  - 0264-6021 (Print)
IS  - 0264-6021 (Linking)
VI  - 383
IP  - Pt. 3
DP  - 2004 Nov 1
TI  - Characterization of a novel low-molecular-mass dual-specificity phosphatase-3 
      (LDP-3) that enhances activation of JNK and p38.
PG  - 447-55
AB  - We have isolated a mouse cDNA for a novel dual-specificity phosphatase designated 
      LDP-3 (low-molecular-mass dual-specificity phosphatase 3). The 450 bp open 
      reading frame encodes a protein of 150 amino acids with a predicted molecular 
      mass of 16 kDa. Northern blot and reverse transcription-PCR analyses show that 
      LDP-3 transcripts are expressed in almost all mouse tissues examined. In vitro 
      analyses using several substrates and inhibitors indicate that LDP-3 possesses 
      intrinsic dual-specificity phosphatase activity. When expressed in mammalian 
      cells, LDP-3 protein is localized mainly to the apical submembrane area. Forced 
      expression of LDP-3 does not alter activation of ERK 
      (extracellular-signal-regulated kinase), but rather enhances activation of JNK 
      (c-Jun N-terminal kinase) and p38 and their respective upstream kinases MKK4 
      (mitogen-activated protein kinase kinase 4) and MKK6 in cells treated with 0.4 M 
      sorbitol. By screening with a variety of stimuli, we found that LDP-3 
      specifically enhances the osmotic stress-induced activation of JNK and p38.
FAU - Takagaki, Kentaro
AU  - Takagaki K
AD  - Division of Biochemical Oncology and Immunology, Institute for Genetic Medicine, 
      Hokkaido University, Kita-15, Nishi-7, Kita-ku, Sapporo 060-0815, Japan.
FAU - Satoh, Takeshi
AU  - Satoh T
FAU - Tanuma, Nobuhiro
AU  - Tanuma N
FAU - Masuda, Kouhei
AU  - Masuda K
FAU - Takekawa, Mutsuhiro
AU  - Takekawa M
FAU - Shima, Hiroshi
AU  - Shima H
FAU - Kikuchi, Kunimi
AU  - Kikuchi K
LA  - eng
SI  - GENBANK/AB164404
SI  - GENBANK/AB183013
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 6)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.25 (MAP Kinase Kinase Kinase 4)
RN  - EC 3.1.3.48 (Dual-Specificity Phosphatases)
RN  - EC 3.1.3.48 (Dusp23 protein, mouse)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - COS Cells/enzymology
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Dual-Specificity Phosphatases
MH  - Enzyme Activation/genetics/physiology
MH  - Humans
MH  - JNK Mitogen-Activated Protein Kinases/*metabolism
MH  - MAP Kinase Kinase Kinase 4/metabolism
MH  - Mice
MH  - Mitogen-Activated Protein Kinase 6/metabolism
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Mutation/genetics/physiology
MH  - Osmotic Pressure
MH  - Protein Tyrosine Phosphatases/*chemistry/genetics/*physiology
MH  - Substrate Specificity
MH  - Transfection/methods
MH  - p38 Mitogen-Activated Protein Kinases/*metabolism
PMC - PMC1133737
EDAT- 2004/07/30 05:00
MHDA- 2005/05/12 09:00
PMCR- 2005/05/01
CRDT- 2004/07/30 05:00
PHST- 2004/07/30 05:00 [pubmed]
PHST- 2005/05/12 09:00 [medline]
PHST- 2004/07/30 05:00 [entrez]
PHST- 2005/05/01 00:00 [pmc-release]
AID - BJ20040498 [pii]
AID - bj3830447 [pii]
AID - 10.1042/BJ20040498 [doi]
PST - ppublish
SO  - Biochem J. 2004 Nov 1;383(Pt. 3):447-55. doi: 10.1042/BJ20040498.