PMID- 15282092
OWN - NLM
STAT- MEDLINE
DCOM- 20050401
LR  - 20191210
IS  - 1570-0232 (Print)
IS  - 1570-0232 (Linking)
VI  - 809
IP  - 1
DP  - 2004 Sep 25
TI  - Automated solid-phase extraction method for the determination of piperaquine in 
      capillary blood applied onto sampling paper by liquid chromatography.
PG  - 43-9
AB  - A bioanalytical method for the determination of piperaquine in 100 microL blood 
      applied onto sampling paper, by solid-phase extraction and liquid chromatography, 
      has been developed and validated. Blood spots were cut into small pieces prior to 
      addition of 0.3M perchloric acid, acetonitrile and phosphate buffer containing an 
      internal standard. The liquid phase was loaded onto a mixed phase cation-exchange 
      (MPC) solid-phase extraction column. Piperaquine and the internal standard were 
      analysed by liquid chromatography and separated on a Chromolith Performance (100 
      mm x 4.6 mm) column with acetonitrile:phosphate buffer pH 2.5, I = 0.1 (8:92, 
      v/v) at the flow of 3.5 mL/min. The UV detection was performed at 345 nm. The 
      intra-assay precision was 12.0% at 0.150 microM, 7.3% at 1.25 microM and 7.3% at 
      2.25 microM. The inter-assay precision was 1.8% at 0.150 microM, 5.2% at 1.25 
      microM and 2.8% at 2.25 microM. The lower limit of quantification (LLOQ) was 
      determined to 0.050 microM where the precision was 14.7%.
FAU - Malm, M
AU  - Malm M
AD  - Dalarna University College, S-781 88, Borlange, Sweden.
FAU - Lindegardh, N
AU  - Lindegardh N
FAU - Bergqvist, Y
AU  - Bergqvist Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Antimalarials)
RN  - 0 (Quinolines)
RN  - A0HV2Q956Y (piperaquine)
SB  - IM
MH  - Antimalarials/*blood
MH  - Calibration
MH  - Chromatography, Liquid/*methods
MH  - Humans
MH  - Quinolines/*blood
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Spectrophotometry, Ultraviolet
EDAT- 2004/07/30 05:00
MHDA- 2005/04/02 09:00
CRDT- 2004/07/30 05:00
PHST- 2003/12/15 00:00 [received]
PHST- 2004/05/17 00:00 [revised]
PHST- 2004/05/28 00:00 [accepted]
PHST- 2004/07/30 05:00 [pubmed]
PHST- 2005/04/02 09:00 [medline]
PHST- 2004/07/30 05:00 [entrez]
AID - S1570023204004672 [pii]
AID - 10.1016/j.jchromb.2004.05.032 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Sep 25;809(1):43-9. doi: 
      10.1016/j.jchromb.2004.05.032.