PMID- 15284880
OWN - NLM
STAT- MEDLINE
DCOM- 20050207
LR  - 20161020
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 47
IP  - 4
DP  - 2004 Aug
TI  - A direct repeat sequence associated with the centromeric retrotransposons in 
      wheat.
PG  - 747-56
AB  - A high-density BAC filter of Triticum monococcum was screened for the presence of 
      a centromeric retrotransposon using the integrase region as a probe. Southern 
      hybridization to the BAC digests using total genomic DNA probes of Triticum 
      monococcum, Triticum aestivum, and Hordeum vulgare detected differentially 
      hybridizing restriction fragments between wheat and barley. The fragments that 
      hybridized to genomic DNA of wheat but not to that of barley were subcloned. 
      Fluorescence in situ hybridization (FISH) analysis indicated that the clone 
      pHind258 hybridized strongly to centromeric regions in wheat and rye and weakly 
      to those in barley. The sequence of pHind258 was homologous to integrase and long 
      terminal repeats of centromeric Ty3-gypsy retrotransposons of cereal species. 
      Additionally, pHind258 has a pair of 192-bp direct repeats. FISH analysis 
      indicated that the 192-bp repeat probe hybridized to centromeres of wheat and rye 
      but not to those of barley. We found differential FISH signal intensities among 
      wheat chromosomes using the 192-bp probe. In general, the A-genome chromosomes 
      possess strong FISH signals, the B-genome chromosomes possess moderate signals, 
      and the D-genome chromosomes possess weak signals. This was consistent with the 
      estimated copy numbers of the 192-bp repeats in the ancestral species of 
      hexaploid wheat.
FAU - Ito, Hidetaka
AU  - Ito H
AD  - Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University, 
      Kitashirakawaoiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan.
FAU - Nasuda, Shuhei
AU  - Nasuda S
FAU - Endo, Takashi R
AU  - Endo TR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (DNA, Plant)
RN  - 0 (Retroelements)
SB  - IM
EIN - Genome. 2004 Oct;47(5):998
MH  - Base Sequence
MH  - Centromere/genetics
MH  - Chromosomes, Artificial, Bacterial/genetics
MH  - Cloning, Molecular
MH  - DNA, Plant/*genetics
MH  - Genome, Plant
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Poaceae/genetics
MH  - *Repetitive Sequences, Nucleic Acid
MH  - Retroelements/*genetics
MH  - Sequence Homology, Nucleic Acid
MH  - Triticum/*genetics
EDAT- 2004/07/31 05:00
MHDA- 2005/02/08 09:00
CRDT- 2004/07/31 05:00
PHST- 2004/07/31 05:00 [pubmed]
PHST- 2005/02/08 09:00 [medline]
PHST- 2004/07/31 05:00 [entrez]
AID - g04-034 [pii]
AID - 10.1139/g04-034 [doi]
PST - ppublish
SO  - Genome. 2004 Aug;47(4):747-56. doi: 10.1139/g04-034.