PMID- 15284882
OWN - NLM
STAT- MEDLINE
DCOM- 20050207
LR  - 20231213
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 47
IP  - 4
DP  - 2004 Aug
TI  - Segmental duplications within the Glycine max genome revealed by fluorescence in 
      situ hybridization of bacterial artificial chromosomes.
PG  - 764-8
AB  - Soybean (Glycine max L. Merr.) is presumed to be an ancient polyploid based on 
      chromosome number and multiple RFLP fragments in genetic mapping. Direct 
      cytogenetic observation of duplicated regions within the soybean genome has not 
      heretofore been reported. Employing fluorescence in situ hybridization (FISH) of 
      genetically anchored bacterial artificial chromosomes (BACs) in soybean, we were 
      able to observe that the distal ends of molecular linkage group E had duplicated 
      regions on linkage groups A2 and B2. Further, using fiber-FISH, it was possible 
      to measure the molecular size and organization of one of the duplicated regions. 
      As FISH did not require repetitive DNA for blocking fluorescence signals, we 
      assume that the 200-kb genome region is relatively low in repetitive sequences. 
      This observation, along with the observation that the BACs are located in distal 
      euchromatin regions, has implications for genome structure/evolution and the 
      approach used to sequence the soybean genome.
FAU - Pagel, Janice
AU  - Pagel J
AD  - Department of Agronomy, Purdue University, 915 W. State Street, West Lafayette, 
      IN 47907, USA.
FAU - Walling, Jason G
AU  - Walling JG
FAU - Young, Nevin D
AU  - Young ND
FAU - Shoemaker, Randy C
AU  - Shoemaker RC
FAU - Jackson, Scott A
AU  - Jackson SA
LA  - eng
PT  - Journal Article
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
SB  - IM
MH  - Biological Evolution
MH  - Chromosomes, Artificial, Bacterial/genetics
MH  - Chromosomes, Plant/genetics
MH  - *Gene Duplication
MH  - Genetic Linkage
MH  - *Genome, Plant
MH  - In Situ Hybridization, Fluorescence
MH  - Polymorphism, Restriction Fragment Length
MH  - Glycine max/*genetics
EDAT- 2004/07/31 05:00
MHDA- 2005/02/08 09:00
CRDT- 2004/07/31 05:00
PHST- 2004/07/31 05:00 [pubmed]
PHST- 2005/02/08 09:00 [medline]
PHST- 2004/07/31 05:00 [entrez]
AID - g04-025 [pii]
AID - 10.1139/g04-025 [doi]
PST - ppublish
SO  - Genome. 2004 Aug;47(4):764-8. doi: 10.1139/g04-025.