PMID- 15286406
OWN - NLM
STAT- MEDLINE
DCOM- 20090624
LR  - 20190917
IS  - 0250-5991 (Print)
IS  - 0250-5991 (Linking)
VI  - 29
IP  - 1
DP  - 2004 Mar
TI  - Protective effects of sodium orthovanadate in diabetic reticulocytes and ageing 
      red blood cells of Wistar rats.
PG  - 73-9
AB  - The reticulocytes and the ageing red blood cells (RBCs) namely young (Y), 
      middle-aged (M) and old RBCs (O) of female Wistar rats from different groups such 
      as control animals (C), controls treated with vanadate (C + V), alloxan-induced 
      diabetic (D), diabetic-treated with insulin (D + I) and vanadate (D + V), were 
      fractionated on a percoll/BSA gradient. The following enzymes were measured - 
      hexokinase (HK), glutathione peroxidase (GSH-Px), glutathione reductase (GSSG-R), 
      glutathione-s-transferase (GST), alanine aminotransferase (AlaAT), aspartate 
      aminotransferase (AsAT) and arginase in the hemolysates of all the RBCs 
      fractions. Decreases in the activity of HK and AsAT by about 70%, arginase and 
      GSH-Px by 30% in old RBCs were observed in comparison to reticulocytes of control 
      animals. Increases in the activity of GSSG-R by 86%, AlaAT by more than 400% and 
      GST by 70% were observed in old RBCs in comparison to reticulocytes of control 
      animals. Alloxan diabetic animals showed a further decrease in the activities of 
      HK in Y RBCs by 37%, M RBCs by 39% and O RBCs by 32%, GSH-Px activity in Y RBCs 
      by 13%, M RBCs by 20% and O RBCs by 33% and GST activity in Y RBCs by 14%, M RBCs 
      by 42% and O RBCs by 60% in comparison to their corresponding cells of control 
      animals. An increase in the activity of all the enzymes studied was also observed 
      in reticulocytes of diabetic animals in comparison to reticulocytes of controls. 
      The GSSG-R activity was found to be increased in Y RBCs by 49%, M RBCs by 67% and 
      O RBCs by 64% as compared to the corresponding age-matched cells of control 
      animals. The activity of arginase also decreased in Y RBCs by about10%, M RBCs by 
      20% and O RBCs by 30% in comparison to the age-matched cells of control animals. 
      A decrease in the activity of AsAT in Y and M RBCs by 30%, and O RBCs by 25% was 
      observed in diabetic animals in comparison to the age-matched cells of control 
      animals. The activity of AlaAT was found to be decreased by more than 10% in Y 
      and M RBCs and 25% in O RBCs of diabetic animals in comparison to the age-matched 
      cells of control animals. Insulin administration to diabetic animals reversed the 
      altered enzyme activity to control values. Vanadate treatment also reversed the 
      enzyme levels except for that of GST in old cells.
FAU - Gupta, Bihari L
AU  - Gupta BL
AD  - Hormone and Drug Research Laboratory, School of Life Sciences, Jawaharlal Nehru 
      University, New Delhi, India.
FAU - Preet, Anju
AU  - Preet A
FAU - Baquer, Najma Z
AU  - Baquer NZ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - India
TA  - J Biosci
JT  - Journal of biosciences
JID - 8100809
RN  - 0 (Blood Glucose)
RN  - 0 (Hypoglycemic Agents)
RN  - 0 (Insulin)
RN  - 3WHH0066W5 (Vanadates)
SB  - IM
MH  - Animals
MH  - Blood Glucose/drug effects
MH  - Cellular Senescence/*drug effects
MH  - Diabetes Mellitus, Experimental/*blood/chemically induced/drug therapy
MH  - Erythrocytes/*drug effects/enzymology
MH  - Female
MH  - Hypoglycemic Agents/therapeutic use
MH  - Insulin/therapeutic use
MH  - Rats
MH  - Rats, Wistar
MH  - Reticulocytes/*drug effects/enzymology
MH  - Vanadates/*pharmacology
EDAT- 2004/08/03 05:00
MHDA- 2009/06/25 09:00
CRDT- 2004/08/03 05:00
PHST- 2004/08/03 05:00 [pubmed]
PHST- 2009/06/25 09:00 [medline]
PHST- 2004/08/03 05:00 [entrez]
AID - 10.1007/BF02702564 [doi]
PST - ppublish
SO  - J Biosci. 2004 Mar;29(1):73-9. doi: 10.1007/BF02702564.