PMID- 15286945
OWN - NLM
STAT- MEDLINE
DCOM- 20040916
LR  - 20191210
IS  - 1548-8969 (Print)
IS  - 1548-8969 (Linking)
VI  - 301
IP  - 8
DP  - 2004 Aug 1
TI  - Immunolocalization of Na+/K+-ATPase, carbonic anhydrase II, and vacuolar 
      H+-ATPase in the gills of freshwater adult lampreys, Geotria australis.
PG  - 654-65
AB  - As adults, anadromous lampreys migrate from seawater into freshwater rivers, 
      where they require branchial ion (NaCl) absorption for osmoregulation. In 
      teleosts and elasmobranchs, pharmological, immunohistochemical, and molecular 
      data support roles for Na+/K+-ATPase (NPPase), carbonic anhydrase II (CAII), and 
      vacuolar H+-ATPase (V-ATPase) in two different models of branchial ion 
      absorption. To our knowledge, these transport-related proteins have not been 
      studied in adult freshwater lampreys, and therefore it is not known if they are 
      expressed, or have similar functions, in lampreys. The purpose of this study was 
      to localize NPPase, CAII, and V-ATPase in the gills of adult freshwater lampreys 
      and determine if any of these transport-related proteins are expressed in the 
      same cells. Heterologous antibodies were used to localize the three proteins in 
      gill tissue from pouched lamprey (Geotria australis). Immunoreactivity (IR) for 
      all three proteins occurred between, and at the base of, lamellae in cells that 
      match previous descriptions of mitochondrion-rich-cells (MRCs). NPPase-IR was 
      always on the basolateral side of cells that did not stain for CAII or V-ATPase. 
      In contrast, CAII-IR was always on the apical side of cells that also contained 
      diffuse V-ATPase-IR. Therefore, we have identified two types of MRC in adult 
      freshwater lamprey gills based on immunohistochemical staining for three 
      transport proteins. A model of ion transport, based on our results, is proposed 
      for adult freshwater lampreys.
FAU - Choe, Keith P
AU  - Choe KP
AD  - Department of Zoology, University of Florida, Gainesville, Florida 32611, USA. 
      kchoe@zoo.ufl.edu
FAU - O'Brien, Siobhan
AU  - O'Brien S
FAU - Evans, David H
AU  - Evans DH
FAU - Toop, Tes
AU  - Toop T
FAU - Edwards, Susan L
AU  - Edwards SL
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - J Exp Zool A Comp Exp Biol
JT  - Journal of experimental zoology. Part A, Comparative experimental biology
JID - 101168223
RN  - 0 (Antibodies, Heterophile)
RN  - EC 3.6.1.- (Vacuolar Proton-Translocating ATPases)
RN  - EC 4.2.1.- (Carbonic Anhydrase II)
RN  - EC 7.2.2.13 (Sodium-Potassium-Exchanging ATPase)
SB  - IM
MH  - Animals
MH  - Antibodies, Heterophile
MH  - Biological Transport, Active
MH  - Carbonic Anhydrase II/*metabolism
MH  - Fresh Water
MH  - Gills/*metabolism
MH  - Immunoblotting
MH  - Immunohistochemistry
MH  - Ion Transport
MH  - Lampreys/*metabolism
MH  - *Models, Biological
MH  - Sodium-Potassium-Exchanging ATPase/*metabolism
MH  - Tasmania
MH  - Vacuolar Proton-Translocating ATPases/*metabolism
EDAT- 2004/08/03 05:00
MHDA- 2004/09/17 05:00
CRDT- 2004/08/03 05:00
PHST- 2004/08/03 05:00 [pubmed]
PHST- 2004/09/17 05:00 [medline]
PHST- 2004/08/03 05:00 [entrez]
AID - 10.1002/jez.a.60 [doi]
PST - ppublish
SO  - J Exp Zool A Comp Exp Biol. 2004 Aug 1;301(8):654-65. doi: 10.1002/jez.a.60.