PMID- 15288188
OWN - NLM
STAT- MEDLINE
DCOM- 20050118
LR  - 20061115
IS  - 0165-0378 (Print)
IS  - 0165-0378 (Linking)
VI  - 62
IP  - 1-2
DP  - 2004 Jun
TI  - Comparison of decidual leukocytes following spontaneous vaginal delivery and 
      elective cesarean section in uncomplicated human term pregnancy.
PG  - 125-37
AB  - The aim of this study was to quantify and compare leukocyte populations in term 
      decidua basalis and parietalis obtained after spontaneous vaginal delivery (SVD) 
      or elective cesarean section (CS) without labor. Decidua basalis and parietalis 
      samples were obtained from placentas after SVD (n = 20) and after CS (n = 30). 
      Following mechanical disaggregation, leukocytes were purified and stained with 
      monoclonal antibodies. Percentages of leukocyte subclasses within the CD45(+) 
      cell fraction and activated T cells were determined by flow cytometry. No 
      differences were found in the percentages of CD45(+) cells or CD56(bright)CD16(-) 
      uterine natural killer (NK) cells between decidua basalis from SVD and CS or 
      between decidua parietalis from SVD and CS. In decidua basalis and parietalis 
      from SVD, a significantly higher number of CD56(dim)CD16(+) NK cells was found 
      compared to CS. In decidua basalis from SVD, there was a significantly lower 
      percentage of CD14(+) cells and higher percentage of CD19(+) cells compared to 
      CS. The percentage of CD3(+) T cells expressing CD25 or human leukocyte antigen 
      (HLA)-DR was significantly decreased in decidua basalis and parietalis from SVD 
      compared to CS. Comparison of decidua collected after SVD or CS suggests that 
      labor is associated with dynamic changes in the distribution of decidual 
      leukocytes, specifically NK and T cell subpopulations. In particular, the 
      disappearance of the CD4(+)CD25(+) T cell population, which possibly contains a 
      subpopulation of regulatory T cells, may contribute to the initiation of labor. 
      Further investigation into factors affecting decidual leukocytes may expand our 
      understanding of the immunological events at the maternal-fetal interface.
FAU - Sindram-Trujillo, Aliana P
AU  - Sindram-Trujillo AP
AD  - Department of Obstetrics, Leiden University Medical Center, Albinusdreef 2 K6-27, 
      2333 ZA, Leiden, The Netherlands.
FAU - Scherjon, Sicco A
AU  - Scherjon SA
FAU - van Hulst-van Miert, Paula P
AU  - van Hulst-van Miert PP
FAU - Kanhai, Humphrey H H
AU  - Kanhai HH
FAU - Roelen, Dave L
AU  - Roelen DL
FAU - Claas, Frans H J
AU  - Claas FH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Ireland
TA  - J Reprod Immunol
JT  - Journal of reproductive immunology
JID - 8001906
RN  - 0 (Antigens, CD)
SB  - IM
MH  - Antigens, CD/analysis/metabolism
MH  - *Cesarean Section
MH  - Decidua/cytology/*immunology
MH  - Female
MH  - Humans
MH  - Labor, Obstetric/*immunology
MH  - Leukocytes/*classification/immunology
MH  - Natural Childbirth
MH  - Pregnancy
EDAT- 2004/08/04 05:00
MHDA- 2005/01/19 09:00
CRDT- 2004/08/04 05:00
PHST- 2003/08/04 00:00 [received]
PHST- 2003/11/06 00:00 [accepted]
PHST- 2004/08/04 05:00 [pubmed]
PHST- 2005/01/19 09:00 [medline]
PHST- 2004/08/04 05:00 [entrez]
AID - S0165037804000427 [pii]
AID - 10.1016/j.jri.2003.11.007 [doi]
PST - ppublish
SO  - J Reprod Immunol. 2004 Jun;62(1-2):125-37. doi: 10.1016/j.jri.2003.11.007.