PMID- 15288469
OWN - NLM
STAT- MEDLINE
DCOM- 20050209
LR  - 20061115
IS  - 0168-8278 (Print)
IS  - 0168-8278 (Linking)
VI  - 41
IP  - 2
DP  - 2004 Aug
TI  - Colonization of albumin-producing hepatocytes derived from transplanted F344 rat 
      bone marrow cells in the liver of congenic Nagase's analbuminemic rats.
PG  - 215-21
AB  - BACKGROUND/AIMS: We investigated whether bone marrow cells (BMCs) of normal rats 
      can be transformed in albumin-producing hepatocytes in analbuminemic rat livers. 
      METHODS: BMCs (2 x 10(7)) from F344 rats (F344) were infused via the portal vein 
      into the livers of congenic Nagase's analbuminemic rats (F344alb) immediately 
      after 70% hepatectomy (PH). Alternatively, F344alb were hematopoietically 
      reconstituted with F344 BMCs by whole body irradiation and BMC transplantation 
      before PH. The recipients were examined for albumin positive (alb +) hepatocytes 
      and albumin mRNA in the livers as well as serum albumin levels 4 weeks later. 
      Sry3 in situ hybridization was done for the livers of female F344alb that 
      received male F344 BMCs. RESULTS: Livers of untreated F344alb contained a few 
      single and double alb+hepatocytes, but these did not form clusters after PH. 
      Clusters (>3 alb + hepatocytes) were detected in livers of the recipients which 
      were transplanted with BMCs immediately after PH as well as the reconstituted 
      F344alb with or without PH. Normal albumin mRNA was detected in the recipient 
      livers, and serum albumin levels were increased. Sry3 was identified in the 
      alb+clusters in the female recipients. CONCLUSIONS: Transplanted BMCs from normal 
      rats can increase clusters of albumin-producing hepatocytes within the liver of 
      analbuminemic rats.
FAU - Arikura, Jun
AU  - Arikura J
AD  - Department of Surgery, Asahikawa Medical College, 2-1-1-1, Midorigaoka-Higashi, 
      Asahikawa 078-8510, Japan. arikura@asahikawa-med.ac.jp
FAU - Inagaki, Mitsuhiro
AU  - Inagaki M
FAU - Huiling, Xiang
AU  - Huiling X
FAU - Ozaki, Atsuko
AU  - Ozaki A
FAU - Onodera, Kazuhiko
AU  - Onodera K
FAU - Ogawa, Katsuhiro
AU  - Ogawa K
FAU - Kasai, Shinichi
AU  - Kasai S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Hepatol
JT  - Journal of hepatology
JID - 8503886
RN  - 0 (Albumins)
RN  - 0 (Serum Albumin)
SB  - IM
MH  - Albumins/biosynthesis/*genetics
MH  - Animals
MH  - *Bone Marrow Transplantation
MH  - Cell Aggregation
MH  - Female
MH  - *Gene Deletion
MH  - Hepatocytes/*metabolism/pathology
MH  - Liver/metabolism/pathology/*surgery
MH  - Male
MH  - Metabolism, Inborn Errors/genetics/metabolism/pathology/*surgery
MH  - Rats
MH  - Rats, Inbred F344
MH  - Serum Albumin/*deficiency/metabolism
MH  - Stem Cells/metabolism/pathology
MH  - Tissue Donors
EDAT- 2004/08/04 05:00
MHDA- 2005/02/11 09:00
CRDT- 2004/08/04 05:00
PHST- 2003/03/05 00:00 [received]
PHST- 2004/03/12 00:00 [revised]
PHST- 2004/04/02 00:00 [accepted]
PHST- 2004/08/04 05:00 [pubmed]
PHST- 2005/02/11 09:00 [medline]
PHST- 2004/08/04 05:00 [entrez]
AID - S0168827804001898 [pii]
AID - 10.1016/j.jhep.2004.04.020 [doi]
PST - ppublish
SO  - J Hepatol. 2004 Aug;41(2):215-21. doi: 10.1016/j.jhep.2004.04.020.