PMID- 15288607
OWN - NLM
STAT- MEDLINE
DCOM- 20050215
LR  - 20220311
IS  - 1386-6532 (Print)
IS  - 1386-6532 (Linking)
VI  - 31
IP  - 1
DP  - 2004 Sep
TI  - No detection of parvovirus B19 or herpesvirus DNA in giant cell arteritis.
PG  - 11-5
AB  - BACKGROUND: Compelling arguments exist for a role of infectious agent in giant 
      cell arteritis (GCA). Parvovirus B19 and several herpesviruses have focussed the 
      attention in recent years, but the few studies to date have yielded inconsistent 
      results. OBJECTIVES: To study the relationship between the presence of parvovirus 
      B19 DNA or major known herpesviruses and the histopathological features of GCA. 
      STUDY DESIGN: Between January 1997 and March 2002, 147 consecutive temporal 
      artery biopsies were performed in our center because of a clinical suspicion of 
      GCA. Using polymerase chain reaction (PCR) procedures validated by the World 
      Health Organization and employed routinely by our laboratory, we examined the 
      paraffin-embedded specimens for DNA from parvovirus B19, herpes simplex viruses 
      (HSV) 1 and 2, Epstein-Barr virus (EBV), varicella-zoster virus (VZV), human 
      cytomegalovirus (HCMV), and human herpesvirus 6 (HHV-6). We investigated positive 
      results further with immunohistochemistry studies. RESULTS: Fifty of the 147 
      temporal artery biopsies (34%) showed histological features of GCA. Three 
      biopsies (2.5%) were initially PCR positive for parvovirus B19. None of the 
      herpesvirus PCR assays were positive. Upon repeat testing by both PCR and 
      immunohistochemistry, none of the three initially positive parvovirus B19 assays 
      were confirmed. The results of both positive and negative control assays in these 
      studies validated these findings. We confirmed the presence of amplifiable DNA in 
      the temporal artery biopsy specimens using PCR primers for beta-globin and 
      indoleamine 2,3-dioxygenase (IDO). CONCLUSIONS: The results of our study do not 
      support a role in the etiopathogenesis of GCA for either parvovirus B19 or any of 
      these six herpesviruses.
FAU - Rodriguez-Pla, Alicia
AU  - Rodriguez-Pla A
AD  - Pediatrics Department, Stanley Division of Developmental Neurovirology, Johns 
      Hopkins University, Baltimore, MD 21287-4933, USA. arodri12@jhmi.edu
FAU - Bosch-Gil, Josep A
AU  - Bosch-Gil JA
FAU - Echevarria-Mayo, Juan E
AU  - Echevarria-Mayo JE
FAU - Rossello-Urgell, Jose
AU  - Rossello-Urgell J
FAU - Solans-Laque, Roser
AU  - Solans-Laque R
FAU - Huguet-Redecilla, Pere
AU  - Huguet-Redecilla P
FAU - Stone, John H
AU  - Stone JH
FAU - Vilardell-Tarres, Miquel
AU  - Vilardell-Tarres M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Clin Virol
JT  - Journal of clinical virology : the official publication of the Pan American 
      Society for Clinical Virology
JID - 9815671
RN  - 0 (DNA, Viral)
RN  - 0 (Indoleamine-Pyrrole 2,3,-Dioxygenase)
RN  - 9004-22-2 (Globins)
RN  - EC 1.13.11.11 (Tryptophan Oxygenase)
SB  - IM
MH  - Aged
MH  - Biopsy
MH  - DNA, Viral/*analysis
MH  - Female
MH  - Giant Cell Arteritis/etiology/*virology
MH  - Globins/genetics
MH  - Herpesviridae/genetics/*isolation & purification
MH  - Herpesviridae Infections/virology
MH  - Humans
MH  - Immunohistochemistry
MH  - Indoleamine-Pyrrole 2,3,-Dioxygenase
MH  - Male
MH  - Parvoviridae Infections/virology
MH  - Parvovirus B19, Human/genetics/*isolation & purification
MH  - Polymerase Chain Reaction
MH  - Temporal Arteries/pathology/virology
MH  - Tryptophan Oxygenase/genetics
EDAT- 2004/08/04 05:00
MHDA- 2005/02/16 09:00
CRDT- 2004/08/04 05:00
PHST- 2004/05/10 00:00 [revised]
PHST- 2004/05/11 00:00 [accepted]
PHST- 2004/08/04 05:00 [pubmed]
PHST- 2005/02/16 09:00 [medline]
PHST- 2004/08/04 05:00 [entrez]
AID - S1386653204001489 [pii]
AID - 10.1016/j.jcv.2004.05.003 [doi]
PST - ppublish
SO  - J Clin Virol. 2004 Sep;31(1):11-5. doi: 10.1016/j.jcv.2004.05.003.