PMID- 15288922
OWN - NLM
STAT- MEDLINE
DCOM- 20041021
LR  - 20220317
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 102
IP  - 1-2
DP  - 2004 Aug 19
TI  - The effect of a killed porcine reproductive and respiratory syndrome virus 
      (PRRSV) vaccine treatment on virus shedding in previously PRRSV infected pigs.
PG  - 11-8
AB  - Two experiments were conducted to investigate if virus shedding could be reduced 
      following a killed porcine reproductive and respiratory syndrome virus (PRRSV) 
      vaccination (KV) of PRRSV infected pigs. In experiment 1, PRRSV infected pigs 
      were vaccinated with KV on days 14 and 28 following infection. Viremia and serum 
      neutralizing (SN) antibody were compared to infected pigs with no KV. The second 
      experiment was conducted in an identical manner. In addition to viremia and SN 
      antibody, virus in oropharyngeal scrapings and interferon gamma (IFN-gamma) 
      producing cells were monitored. Magnitude and duration of viremia were not 
      different between KV vaccinated and non-vaccinated groups. No virus was detected 
      in oropharyngeal scraping from any pig, nor was there a difference in the 
      detection of viral RNA. In both experiments, however, increases in SN titer and 
      number of IFN-gamma producing cells were observed. The SN titer was significantly 
      higher in KV vaccinated groups than in non-vaccinated group on days 42 and 42-56 
      following infection in experiments 1 and 2, respectively. The number of IFN-gamma 
      producing cells was slightly higher in KV vaccinated groups than in 
      non-vaccinated group on days 42 and 63. These observations suggest that KV had no 
      effect on virus shedding. However, previously infected pigs responded 
      immunologically to KV, as demonstrated by increases in SN antibody titers and 
      IFN-gamma producing cells.
FAU - Nilubol, D
AU  - Nilubol D
AD  - Department of Veterinary Microbiology and Preventive Medicine, Iowa State 
      University, Ames 50011, USA.
FAU - Platt, K B
AU  - Platt KB
FAU - Halbur, P G
AU  - Halbur PG
FAU - Torremorell, M
AU  - Torremorell M
FAU - Harris, D L
AU  - Harris DL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antibodies, Viral)
RN  - 0 (RNA, Viral)
RN  - 0 (Vaccines, Inactivated)
RN  - 0 (Viral Vaccines)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/blood
MH  - Enzyme-Linked Immunosorbent Assay/veterinary
MH  - Interferon-gamma/metabolism
MH  - Leukocytes, Mononuclear/immunology/virology
MH  - Neutralization Tests/veterinary
MH  - Oropharynx/virology
MH  - Porcine Reproductive and Respiratory Syndrome/blood/immunology/*therapy/*virology
MH  - Porcine respiratory and reproductive syndrome virus/genetics/*immunology
MH  - RNA, Viral/chemistry/genetics
MH  - Random Allocation
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Swine
MH  - Vaccination/*veterinary
MH  - Vaccines, Inactivated/immunology/pharmacology
MH  - Viral Vaccines/immunology/*pharmacology
MH  - Viremia/veterinary
MH  - Virus Shedding
EDAT- 2004/08/04 05:00
MHDA- 2004/10/22 09:00
CRDT- 2004/08/04 05:00
PHST- 2003/05/08 00:00 [received]
PHST- 2004/04/15 00:00 [revised]
PHST- 2004/05/10 00:00 [accepted]
PHST- 2004/08/04 05:00 [pubmed]
PHST- 2004/10/22 09:00 [medline]
PHST- 2004/08/04 05:00 [entrez]
AID - S037811350400197X [pii]
AID - 10.1016/j.vetmic.2004.05.006 [doi]
PST - ppublish
SO  - Vet Microbiol. 2004 Aug 19;102(1-2):11-8. doi: 10.1016/j.vetmic.2004.05.006.