PMID- 15288930
OWN - NLM
STAT- MEDLINE
DCOM- 20041021
LR  - 20141120
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 102
IP  - 1-2
DP  - 2004 Aug 19
TI  - Cloning and purification of the Streptococcus suis serotype 2 
      glyceraldehyde-3-phosphate dehydrogenase and its involvement as an adhesin.
PG  - 87-94
AB  - Streptococcus suis serotype 2 is a swine pathogen responsible for diverse 
      diseases and may be present in the tonsils of pigs which show no sign of illness. 
      Because adhesion to host cells may be important in the carrier state, this study 
      was undertaken to characterize a 39 kDa surface protein identified as a 
      glyceraldehyde-3-phosphate dehydrogenase (GAPDH), possibly implicated in the 
      adhesion of the bacteria. The gene encoding for the GAPDH of S. suis was cloned 
      and sequenced. The DNA sequence contained an open reading frame encoding for a 
      336 amino acid polypeptide exhibiting 95% sequence identity with the GAPDH from 
      Streptococcus pyogenes and from other streptococci. Using the Qiaexpress 
      expression plasmids, the gapdh gene was inducibly overexpressed in E. coli to 
      produce GAPDH with a hexahistidyl N-terminus to permit its purification. The 
      (His)6GAPDH protein was found to possess functional GAPDH enzymatic activity 
      after the purification. An adherence assay with S. suis and porcine tracheal 
      rings pre-incubated with (His)6GAPDH and non-incubated rings was showed a 
      significant reduction in the adhesion of S. suis in the (His)6GAPDH pre-incubated 
      rings compared to the non-incubated rings. The GAPDH protein of S. suis seems to 
      be involved in the first steps of the bacterial adhesion to host cells.
FAU - Brassard, Julie
AU  - Brassard J
AD  - GREMIP, Departement de Pathologie et Microbiologie, Faculte de medecine 
      veterinaire, Universite de Montreal, CP 5000, Saint-Hyacinthe, Quebec, Canada J2S 
      7C6.
FAU - Gottschalk, Marcelo
AU  - Gottschalk M
FAU - Quessy, Sylvain
AU  - Quessy S
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (DNA, Bacterial)
RN  - 0 (Recombinant Proteins)
RN  - EC 1.2.1.9 (Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+))
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Bacterial Adhesion/*physiology
MH  - Base Sequence
MH  - Chromatography, Affinity/veterinary
MH  - Cloning, Molecular
MH  - DNA, Bacterial/chemistry/genetics
MH  - Electrophoresis, Polyacrylamide Gel/veterinary
MH  - Glyceraldehyde 3-Phosphate Dehydrogenase (NADP+)/*genetics/isolation & 
      purification/*pharmacology
MH  - In Vitro Techniques
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction/veterinary
MH  - Recombinant Proteins/genetics/isolation & purification
MH  - Sequence Alignment
MH  - Streptococcal Infections/microbiology/*veterinary
MH  - Streptococcus suis/*enzymology/genetics
MH  - Swine
MH  - Swine Diseases/*microbiology
MH  - Trachea/*microbiology
EDAT- 2004/08/04 05:00
MHDA- 2004/10/22 09:00
CRDT- 2004/08/04 05:00
PHST- 2003/08/04 00:00 [received]
PHST- 2004/05/13 00:00 [revised]
PHST- 2004/05/14 00:00 [accepted]
PHST- 2004/08/04 05:00 [pubmed]
PHST- 2004/10/22 09:00 [medline]
PHST- 2004/08/04 05:00 [entrez]
AID - S0378113504002068 [pii]
AID - 10.1016/j.vetmic.2004.05.008 [doi]
PST - ppublish
SO  - Vet Microbiol. 2004 Aug 19;102(1-2):87-94. doi: 10.1016/j.vetmic.2004.05.008.