PMID- 15288957
OWN - NLM
STAT- MEDLINE
DCOM- 20041021
LR  - 20210119
IS  - 0166-0934 (Print)
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 120
IP  - 2
DP  - 2004 Sep 15
TI  - Detection of economically important viruses in boar semen by quantitative 
      RealTime PCR technology.
PG  - 151-60
AB  - The objective of this study was to develop quantitative real-time polymerase 
      chain reaction (ReTi-PCR) tests for the detection of five economically important 
      viruses in swine semen namely, pseudorabies virus (PRV), classical swine fever 
      virus (CSFV), foot-and-mouth disease virus (FMDV), swine vesicular disease virus 
      (SVDV), and porcine reproductive and respiratory syndrome virus (PRRSV). Each 
      ReTi-PCR test was validated for specificity, analytical sensitivity (detection 
      limits), and experimental infection studies were performed to compare the 
      conventional virus isolation methods with the newly developed ReTi-PCR tests. All 
      five developed ReTi-PCR tests are very rapid compared to virus isolation, highly 
      specific, and even more sensitive (lower detection limits) than conventional 
      virus isolation methods for the detection of mentioned viruses in semen. In semen 
      of experimentally infected boars, viruses were detected much earlier after 
      infection and more frequently by ReTi-PCR tests than by virus isolations. The 
      high throughput of these rapid ReTi-PCR tests makes it possible to screen large 
      number of semen samples for the presence of viruses prior to insemination. This 
      is a substantial advantage, in particular for boar semen the quality of which 
      deteriorates quickly after storage. In general, the newly developed ReTi-PCR 
      tests are valuable tools for the early, reliable and rapid detection of five 
      economically important viruses, namely PRV, CSFV, FMDV, SVDV, and PRRSV in boar 
      semen. These ReTi-PCR tests will improve the control of viral diseases 
      transmitted via semen.
FAU - van Rijn, Piet A
AU  - van Rijn PA
AD  - Central Institute for Animal Disease Control (CIDC-Lelystad), P.O. Box 2004, 
      Lelystad 8204 AA, The Netherlands.
FAU - Wellenberg, Gerard J
AU  - Wellenberg GJ
FAU - Hakze-van der Honing, Renate
AU  - Hakze-van der Honing R
FAU - Jacobs, Liesbeth
AU  - Jacobs L
FAU - Moonen, Peter L J M
AU  - Moonen PL
FAU - Feitsma, Hanneke
AU  - Feitsma H
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
SB  - IM
MH  - Animals
MH  - Classical Swine Fever Virus/genetics/isolation & purification
MH  - Enterovirus B, Human/genetics/isolation & purification
MH  - Foot-and-Mouth Disease Virus/genetics/isolation & purification
MH  - Herpesvirus 1, Suid/genetics/isolation & purification
MH  - Humans
MH  - Polymerase Chain Reaction/*methods
MH  - Porcine respiratory and reproductive syndrome virus/genetics/isolation & 
      purification
MH  - Semen/*virology
MH  - Swine
MH  - Swine Diseases/economics/*virology
MH  - Time Factors
MH  - Virus Diseases/economics/*veterinary/virology
MH  - Viruses/genetics/*isolation & purification
PMC - PMC7119495
EDAT- 2004/08/04 05:00
MHDA- 2004/10/22 09:00
PMCR- 2004/06/19
CRDT- 2004/08/04 05:00
PHST- 2003/11/27 00:00 [received]
PHST- 2004/04/10 00:00 [revised]
PHST- 2004/04/14 00:00 [accepted]
PHST- 2004/08/04 05:00 [pubmed]
PHST- 2004/10/22 09:00 [medline]
PHST- 2004/08/04 05:00 [entrez]
PHST- 2004/06/19 00:00 [pmc-release]
AID - S0166093404001326 [pii]
AID - 10.1016/j.jviromet.2004.04.014 [doi]
PST - ppublish
SO  - J Virol Methods. 2004 Sep 15;120(2):151-60. doi: 10.1016/j.jviromet.2004.04.014.