PMID- 15291345
OWN - NLM
STAT- MEDLINE
DCOM- 20041230
LR  - 20190116
IS  - 1042-8194 (Print)
IS  - 1026-8022 (Linking)
VI  - 45
IP  - 5
DP  - 2004 May
TI  - Allelotyping in B-cell chronic lymphocytic leukemia (B-CLL).
PG  - 887-96
AB  - B-cell chronic lymphocytic leukemia (B-CLL) is a heterogeneous malignant disease, 
      both in terms of molecular abnormalities and clinical course. The most frequent 
      chromosomal aberrations in B-CLL are deletions on 13q, 11q, and 17p, and trisomy 
      12, all of which are of prognostic significance. These aberrations can be 
      detected by conventional cytogenetic analysis and fluorescence in situ 
      hybridization (FISH), but cytogenetics are hampered by the low mitotic index of 
      B-CLL cells, and FISH depends on genetic information of candidate regions. 
      Microsatellites are unique highly polymorphic and informative genetic markers 
      dispersed in the human genome. They have become the most commonly used markers to 
      trace loss of heterozygosity in tumors. Their detection by PCR is rapid and can 
      be semi-automated with maximal robustness and reproducibility. In this review, we 
      discuss the implications of a recent genome-wide analysis in B-CLL with 400 
      microsatellite markers. This analysis led to the detection of new aberrant loci 
      in B-CLL which are not visible in the leukemic conventional karyotype. We 
      conclude that microsatellite allelotyping provides a complementary comprehensive 
      view of genetic alterations in B-CLL, and it may identify new loci with candidate 
      genes relevant in the molecular biology of B-CLL.
FAU - Novak, Urban
AU  - Novak U
AD  - Department of Medical Oncology and Haematology, University and Inselspital Berne, 
      Switzerland.
FAU - Tobler, Andreas
AU  - Tobler A
FAU - Fey, Martin F
AU  - Fey MF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - United States
TA  - Leuk Lymphoma
JT  - Leukemia & lymphoma
JID - 9007422
SB  - IM
MH  - *Alleles
MH  - Chromosome Aberrations
MH  - Cytogenetic Analysis/*methods
MH  - Humans
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics
MH  - Microsatellite Repeats
RF  - 73
EDAT- 2004/08/05 05:00
MHDA- 2004/12/31 09:00
CRDT- 2004/08/05 05:00
PHST- 2004/08/05 05:00 [pubmed]
PHST- 2004/12/31 09:00 [medline]
PHST- 2004/08/05 05:00 [entrez]
AID - 10.1080/1042819032000159843 [doi]
PST - ppublish
SO  - Leuk Lymphoma. 2004 May;45(5):887-96. doi: 10.1080/1042819032000159843.