PMID- 15291356
OWN - NLM
STAT- MEDLINE
DCOM- 20041230
LR  - 20190116
IS  - 1042-8194 (Print)
IS  - 1026-8022 (Linking)
VI  - 45
IP  - 5
DP  - 2004 May
TI  - Detection of chromosome 13q deletions and IgH translocations in patients with 
      multiple myeloma by FISH: comparison with karyotype analysis.
PG  - 965-9
AB  - Multiple myeloma (MM) is a plasma cell dyscrasia characterized by frequent 13q 
      deletions and IgH translocations that have clinical prognostic significance. We 
      evaluated clonal plasma cells by interphase fluorescence in situ hybridization 
      (FISH) and combined with immunofluorescence detection of cytoplasmic light chain 
      (cIg-FISH) for the presence of 13q deletions and IgH translocations. The FISH 
      results were compared with conventional cytogenetic analysis. Of the 25 bone 
      marrow specimens from MM patients, 11 (44%) had 13q deletions. IgH translocations 
      involving cyclin D1 (t(11;14)) and FGFR3 (t(4;14)) were found in 32 and 36%, 
      respectively. P53 deletions were detected in 20% of the cases. One patient had 
      coexistence of t(ll;14) and t(4;14), which has not been previously reported. 
      Conventional cytogenetic analysis was performed in 15 cases and revealed complex 
      numerical and structural changes in 7. Karyotype analysis failed to detect 3 of 6 
      cases with 13q deletions, and also missed most of the IgH translocations and p53 
      deletions detected by cIg-FISH. On the other hand, the complex numerical and 
      structural changes shown by conventional cytogenetics were not demonstrated by 
      interphase FISH. Since 13q deletions, IgH translocations and a hypodiploid 
      karyotype are significant prognostic factors for MM, our study illustrates the 
      importance of combining conventional cytogenetics with interphase FISH analysis 
      in patients with MM.
FAU - Chang, Hong
AU  - Chang H
AD  - Department of Laboratory Hematology, Princess Margaret Hospital, Toronto, Canada. 
      hong.chang@uhn.on.ca
FAU - Li, Dan
AU  - Li D
FAU - Zhuang, Lihua
AU  - Zhuang L
FAU - Nie, Eileen
AU  - Nie E
FAU - Bouman, Derek
AU  - Bouman D
FAU - Stewart, A Keith
AU  - Stewart AK
FAU - Chun, Kathy
AU  - Chun K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Leuk Lymphoma
JT  - Leukemia & lymphoma
JID - 9007422
RN  - 0 (Immunoglobulin Heavy Chains)
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Chromosome Deletion
MH  - *Chromosomes, Human, Pair 13
MH  - Clone Cells/pathology
MH  - Cytogenetic Analysis/*methods/standards
MH  - Female
MH  - Humans
MH  - Immunoglobulin Heavy Chains/*genetics
MH  - In Situ Hybridization, Fluorescence/standards
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Multiple Myeloma/diagnosis/*genetics
MH  - Prognosis
MH  - *Translocation, Genetic
EDAT- 2004/08/05 05:00
MHDA- 2004/12/31 09:00
CRDT- 2004/08/05 05:00
PHST- 2004/08/05 05:00 [pubmed]
PHST- 2004/12/31 09:00 [medline]
PHST- 2004/08/05 05:00 [entrez]
AID - 10.1080/10428190310001638832 [doi]
PST - ppublish
SO  - Leuk Lymphoma. 2004 May;45(5):965-9. doi: 10.1080/10428190310001638832.