PMID- 15291877
OWN - NLM
STAT- MEDLINE
DCOM- 20040927
LR  - 20220516
IS  - 1464-4096 (Print)
IS  - 1464-4096 (Linking)
VI  - 94
IP  - 3
DP  - 2004 Aug
TI  - Fluorescence in situ hybridization analysis of matched primary tumour and 
      lymph-node metastasis of D1 (pT2-3pN1M0) prostate cancer.
PG  - 407-11
AB  - OBJECTIVE: To describe the chromosomal numerical changes present in primary 
      prostate tumours and their matched lymph-node metastases, to identify a clonal 
      cell migration process which could account for the metastatic behaviour. 
      MATERIALS AND METHODS: Twenty-eight cases of unsuspected stage D1 (pT2-3pN1M0) 
      prostate cancer were detected among patients who had a radical prostatectomy for 
      clinically localized prostate cancer. Fluorescence in situ hybridization (FISH), 
      using centromeric probes to enumerate chromosomes 7, 8, 10 and 12, was used to 
      assess numerical chromosomal changes. FISH analysis was used on isolated nuclei 
      obtained from matched primary tumours and their lymph node metastases. RESULTS: 
      Of the 28 suitable cases it was possible to complete the study in 18 pairs of 
      matched tissues; the remainder were excluded because of insufficient tissue or 
      poor preservation of at least one of the tissues. There was cytogenetic change 
      (aneuploidy) in 16 of the 18 primary tumours, the most common being monosomy 8, 
      detected in 14, followed by trisomy 7, in 13 aneuploid tumours. All lymph node 
      metastases were aneuploid by FISH. As in the primary tumours, monosomy 8 and 
      trisomy 7 were the most common cytogenetic alterations, in 13 and 15 of the lymph 
      node tissues. FISH analysis showed a high correlation (83%) in the cytogenetic 
      pattern of changes between the primary tumours and their lymph node metastases. 
      Moreover, a similar number of cells had the most common aneusomies when comparing 
      prostate and the lymph node tissues. CONCLUSIONS: These results show a similar 
      pattern of cytogenetic alteration in the primary tumour and its lymph node 
      metastasis, characterized by the frequent presence of trisomy 7 and monosomy 8, 
      suggesting that clonal cell selection is not involved in the metastatic process.
FAU - Alcaraz, Antonio
AU  - Alcaraz A
AD  - Department of Urology, Hospital Clinic, Fundacio Puigvert, C/Cartagena 340-350, 
      08025 Barcelona, Spain. aalcatraz@fundacio-puigvert.es
FAU - Corral, Juan M
AU  - Corral JM
FAU - Ribal, Maria J
AU  - Ribal MJ
FAU - Mallofre, Carme
AU  - Mallofre C
FAU - Mengual, Lourdes
AU  - Mengual L
FAU - Carrio, Ana
AU  - Carrio A
FAU - Gil-Vernet Sedo, Jose Maria
AU  - Gil-Vernet Sedo JM
FAU - Villavicencio, Humberto
AU  - Villavicencio H
LA  - eng
PT  - Journal Article
PL  - England
TA  - BJU Int
JT  - BJU international
JID - 100886721
SB  - IM
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human/*genetics
MH  - Humans
MH  - In Situ Hybridization
MH  - Lymphatic Metastasis/*genetics
MH  - Male
MH  - Prostatic Neoplasms/*genetics
EDAT- 2004/08/05 05:00
MHDA- 2004/09/28 05:00
CRDT- 2004/08/05 05:00
PHST- 2004/08/05 05:00 [pubmed]
PHST- 2004/09/28 05:00 [medline]
PHST- 2004/08/05 05:00 [entrez]
AID - BJU4829 [pii]
AID - 10.1111/j.1464-410X.2004.04829.x [doi]
PST - ppublish
SO  - BJU Int. 2004 Aug;94(3):407-11. doi: 10.1111/j.1464-410X.2004.04829.x.