PMID- 15292518
OWN - NLM
STAT- MEDLINE
DCOM- 20050426
LR  - 20131121
IS  - 1741-0126 (Print)
IS  - 1741-0126 (Linking)
VI  - 17
IP  - 6
DP  - 2004 Jun
TI  - The pyruvate formate lyase family: sequences, structures and activation.
PG  - 545-52
AB  - We cloned and expressed in Escherichia coli the Archaeglobus fulgidus gene that 
      encodes pyruvate formate lyase 2 (PFL2). PFL2, despite its homology to the other 
      glycyl radical enzymes, differs from them by exhibiting a completely different 
      oligomerization. The most abundant form of PFL2 when expressed in E.coli is a 
      trimer. The closest homologue of PFL2 with a known structure is E. coli PFL, 
      which is a dimer. Sequence comparisons allowed us to reclassify PFL-like enzymes 
      and the consensus sequences allowed us to propose an activation route for 
      PFL-like glycyl radical enzymes. Surprisingly, most of the conserved residues in 
      PFL-like enzymes appear to be involved in preserving the structure, rather than 
      forming the active site.
FAU - Lehtio, L
AU  - Lehtio L
AD  - Graduate School in Informational and Structural Biology, Institute of 
      Biotechnology, University of Helsinki, PO Box 65, FIN-00014 Helsinki, Finland.
FAU - Goldman, A
AU  - Goldman A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040803
PL  - England
TA  - Protein Eng Des Sel
JT  - Protein engineering, design & selection : PEDS
JID - 101186484
RN  - 0 (DNA, Archaeal)
RN  - 0 (Protein Subunits)
RN  - 0 (Recombinant Proteins)
RN  - EC 2.3.1.- (Acetyltransferases)
RN  - EC 2.3.1.54 (formate C-acetyltransferase)
RN  - K848JZ4886 (Cysteine)
SB  - IM
MH  - Acetyltransferases/chemistry/classification/*genetics
MH  - Amino Acid Sequence
MH  - Archaeoglobus fulgidus/*enzymology/genetics
MH  - Catalytic Domain/genetics
MH  - Chromatography, Gel
MH  - Cloning, Molecular
MH  - Consensus Sequence/genetics
MH  - Conserved Sequence/genetics
MH  - Cysteine/genetics
MH  - DNA, Archaeal/chemistry/genetics
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Enzyme Activation
MH  - Escherichia coli/genetics
MH  - Light
MH  - Models, Molecular
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Phylogeny
MH  - Protein Structure, Tertiary
MH  - Protein Subunits/chemistry/genetics
MH  - Recombinant Proteins/biosynthesis/chemistry/isolation & purification
MH  - Scattering, Radiation
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
EDAT- 2004/08/05 05:00
MHDA- 2005/04/27 09:00
CRDT- 2004/08/05 05:00
PHST- 2004/08/05 05:00 [pubmed]
PHST- 2005/04/27 09:00 [medline]
PHST- 2004/08/05 05:00 [entrez]
AID - gzh059 [pii]
AID - 10.1093/protein/gzh059 [doi]
PST - ppublish
SO  - Protein Eng Des Sel. 2004 Jun;17(6):545-52. doi: 10.1093/protein/gzh059. Epub 
      2004 Aug 3.