PMID- 15294780
OWN - NLM
STAT- MEDLINE
DCOM- 20040928
LR  - 20181130
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 70
IP  - 8
DP  - 2004 Aug
TI  - Analysis of structural and physiological profiles to assess the effects of Cu on 
      biofilm microbial communities.
PG  - 4512-21
AB  - We investigated the effects of copper on the structure and physiology of 
      freshwater biofilm microbial communities. For this purpose, biofilms that were 
      grown during 4 weeks in a shallow, slightly polluted ditch were exposed, in 
      aquaria in our laboratory, to a range of copper concentrations (0, 1, 3, and 10 
      microM). Denaturing gradient gel electrophoresis (DGGE) revealed changes in the 
      bacterial community in all aquaria. The extent of change was related to the 
      concentration of copper applied, indicating that copper directly or indirectly 
      caused the effects. Concomitantly with these changes in structure, changes in the 
      metabolic potential of the heterotrophic bacterial community were apparent from 
      changes in substrate use profiles as assessed on Biolog plates. The structure of 
      the phototrophic community also changed during the experiment, as observed by 
      microscopic analysis in combination with DGGE analysis of eukaryotic 
      microorganisms and cyanobacteria. However, the extent of community change, as 
      observed by DGGE, was not significantly greater in the copper treatments than in 
      the control. Yet microscopic analysis showed a development toward a greater 
      proportion of cyanobacteria in the treatments with the highest copper 
      concentrations. Furthermore, copper did affect the physiology of the phototrophic 
      community, as evidenced by the fact that a decrease in photosynthetic capacity 
      was detected in the treatment with the highest copper concentration. Therefore, 
      we conclude that copper affected the physiology of the biofilm and had an effect 
      on the structure of the communities composing this biofilm.
FAU - Massieux, B
AU  - Massieux B
AD  - Netherlands Institute of Ecology, Centre for Limnology, Rijkstraatweg 6, 3631 AC 
      Nieuwersluis, The Netherlands. b.massieux@nioo.knaw.nl
FAU - Boivin, M E Y
AU  - Boivin ME
FAU - Van Den Ende, F P
AU  - Van Den Ende FP
FAU - Langenskiold, J
AU  - Langenskiold J
FAU - Marvan, P
AU  - Marvan P
FAU - Barranguet, C
AU  - Barranguet C
FAU - Admiraal, W
AU  - Admiraal W
FAU - Laanbroek, H J
AU  - Laanbroek HJ
FAU - Zwart, G
AU  - Zwart G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 1406-65-1 (Chlorophyll)
RN  - 789U1901C5 (Copper)
RN  - YF5Q9EJC8Y (Chlorophyll A)
SB  - IM
MH  - Bacteria/*drug effects/growth & development
MH  - Biofilms/*drug effects/growth & development
MH  - Biomass
MH  - Chlorophyll/metabolism
MH  - Chlorophyll A
MH  - Copper/*pharmacology
MH  - Ecosystem
MH  - Electrophoresis/methods
MH  - Eukaryota/*drug effects/growth & development
MH  - Fluorescence
MH  - Fresh Water/*microbiology
MH  - Microbiological Techniques
PMC - PMC492431
EDAT- 2004/08/06 05:00
MHDA- 2004/09/29 05:00
PMCR- 2004/08/01
CRDT- 2004/08/06 05:00
PHST- 2004/08/06 05:00 [pubmed]
PHST- 2004/09/29 05:00 [medline]
PHST- 2004/08/06 05:00 [entrez]
PHST- 2004/08/01 00:00 [pmc-release]
AID - 70/8/4512 [pii]
AID - 2207-03 [pii]
AID - 10.1128/AEM.70.8.4512-4521.2004 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2004 Aug;70(8):4512-21. doi: 
      10.1128/AEM.70.8.4512-4521.2004.