PMID- 15294798
OWN - NLM
STAT- MEDLINE
DCOM- 20040928
LR  - 20181113
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 70
IP  - 8
DP  - 2004 Aug
TI  - Use of microautoradiography combined with fluorescence in situ hybridization to 
      determine dimethylsulfoniopropionate incorporation by marine bacterioplankton 
      taxa.
PG  - 4648-57
AB  - The fraction of planktonic heterotrophic bacteria capable of incorporating 
      dissolved dimethylsulfoniopropionate (DMSP) and leucine was determined at two 
      coastal sites by microautoradioagraphy (AU). In Gulf of Mexico seawater microcosm 
      experiments, the proportion of prokaryotes that incorporated sulfur from 
      [(35)S]DMSP ranged between 27 and 51% of 4',6-diamidino-2-phenylindole 
      (DAPI)-positive cells, similar to or slightly lower than the proportion 
      incorporating [(3)H]leucine. In the northwest Mediterranean coast, the proportion 
      of cells incorporating sulfur from [(35)S]DMSP increased from 5 to 42% from 
      January to March, coinciding with the development of a phytoplankton bloom. At 
      the same time, the proportion of cells incorporating [(3)H]leucine increased from 
      21 to 40%. The combination of AU and fluorescence in situ hybridization (FISH) 
      revealed that the Roseobacter clade (alpha-proteobacteria) accounted for 13 to 
      43% of the microorganisms incorporating [(35)S]DMSP at both sampling sites. 
      Significant uptake of sulfur from DMSP was also found among members of the 
      gamma-proteobacteria and Cytophaga-Flavobacterium groups. Roseobacter and 
      gamma-proteobacteria exhibited the highest percentage of DAPI-positive cells 
      incorporating (35)S from DMSP (around 50%). Altogether, the application of AU 
      with [(35)S]DMSP combined with FISH indicated that utilization of S from DMSP is 
      a widespread feature among active marine bacteria, comparable to leucine 
      utilization. These results point toward DMSP as an important substrate for a 
      broad and diverse fraction of marine bacterioplankton.
FAU - Vila, Maria
AU  - Vila M
AD  - Institut de Ciencies del Mar-CMIMA, Passeig Maritim de la Barceloneta 37-49, 
      08003 Barcelona, Catalonia, Spain.
FAU - Simo, Rafel
AU  - Simo R
FAU - Kiene, Ronald P
AU  - Kiene RP
FAU - Pinhassi, Jarone
AU  - Pinhassi J
FAU - Gonzalez, Jose M
AU  - Gonzalez JM
FAU - Moran, Mary Ann
AU  - Moran MA
FAU - Pedros-Alio, Carlos
AU  - Pedros-Alio C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (Sulfonium Compounds)
RN  - 0 (Sulfur Radioisotopes)
RN  - C884XA7QGG (dimethylpropiothetin)
SB  - IM
MH  - Animals
MH  - Autoradiography
MH  - Gram-Negative Bacteria/classification/genetics/*metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Plankton/*metabolism
MH  - Seawater/*microbiology
MH  - Sulfonium Compounds/*metabolism
MH  - Sulfur Radioisotopes/metabolism
PMC - PMC492427
EDAT- 2004/08/06 05:00
MHDA- 2004/09/29 05:00
PMCR- 2004/08/01
CRDT- 2004/08/06 05:00
PHST- 2004/08/06 05:00 [pubmed]
PHST- 2004/09/29 05:00 [medline]
PHST- 2004/08/06 05:00 [entrez]
PHST- 2004/08/01 00:00 [pmc-release]
AID - 70/8/4648 [pii]
AID - 2161-03 [pii]
AID - 10.1128/AEM.70.8.4648-4657.2004 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 2004 Aug;70(8):4648-57. doi: 
      10.1128/AEM.70.8.4648-4657.2004.