PMID- 15295891
OWN - NLM
STAT- MEDLINE
DCOM- 20041015
LR  - 20190729
IS  - 1060-3271 (Print)
IS  - 1060-3271 (Linking)
VI  - 87
IP  - 4
DP  - 2004 Jul-Aug
TI  - Solid-phase extraction and cleanup procedures for determination of acrylamide in 
      fried potato products by liquid chromatography/mass spectrometry.
PG  - 961-4
AB  - In response to recent discoveries of acrylamide in heated foods, a solid-phase 
      extraction and cleanup protocol was developed for the determination of acrylamide 
      in fried or baked potato samples by liquid chromatography/mass spectrometry 
      (LC/MS). The analyte was extracted from the matrix by using 2M NaCl, and an 
      aliquot of the initial extract was loaded onto a reversed-phase cartridge. After 
      the analyte was eluted from the cartridge, the eluate was cleaned up on a 
      mixed-mode cation-exchange cartridge. The eluate was then evaporated, and the 
      residue was reconstituted in mobile phase before LC/MS analysis. Recoveries, 
      based on the recovery of an added internal standard, ranged from 96 to 101% with 
      relative standard deviations (RSDs) of 5-11%. The response was linear for a 
      concentration range of 100-2000 ng/g with a coefficient of determination (R2) of 
      0.992 (n = 25). An interday study showed good accuracy and precision of the 
      method over a 3-day period with a recovery of 98% and an RSD of 9.5% (n = 15). 
      The analyses of 6 potato chip samples showed concentrations of incurred 
      acrylamide ranging from 260 to 1500 ng/g.
FAU - Young, Michael S
AU  - Young MS
AD  - Waters Corp., Milford, MA 01757, USA. michael_s_young@waters.com
FAU - Jenkins, Kevin M
AU  - Jenkins KM
FAU - Mallet, Claude R
AU  - Mallet CR
LA  - eng
PT  - Journal Article
PL  - England
TA  - J AOAC Int
JT  - Journal of AOAC International
JID - 9215446
RN  - 0 (Acrylamides)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Solvents)
SB  - IM
MH  - Acrylamides/*analysis
MH  - Chromatography, Ion Exchange
MH  - Chromatography, Liquid
MH  - Cooking
MH  - Indicators and Reagents
MH  - Mass Spectrometry
MH  - Reference Standards
MH  - Reproducibility of Results
MH  - Solanum tuberosum/*chemistry
MH  - Solvents
EDAT- 2004/08/07 05:00
MHDA- 2004/10/16 09:00
CRDT- 2004/08/07 05:00
PHST- 2004/08/07 05:00 [pubmed]
PHST- 2004/10/16 09:00 [medline]
PHST- 2004/08/07 05:00 [entrez]
PST - ppublish
SO  - J AOAC Int. 2004 Jul-Aug;87(4):961-4.