PMID- 15297261
OWN - NLM
STAT- MEDLINE
DCOM- 20050308
LR  - 20211203
IS  - 0193-1857 (Print)
IS  - 0193-1857 (Linking)
VI  - 288
IP  - 1
DP  - 2005 Jan
TI  - The gene transfer of soluble VEGF type I receptor (Flt-1) attenuates peritoneal 
      fibrosis formation in mice but not soluble TGF-beta type II receptor gene 
      transfer.
PG  - G143-50
AB  - Peritoneal fibrosis formation is a consequence of inflammation/injury and a 
      significant medical problem to be solved. The effects of soluble VEGF receptor 
      type I (sFlt-1) gene transfer on experimental peritoneal fibrosis were examined 
      and compared with soluble transforming growth factor-beta (TGF-beta) receptor 
      type II (sTGF beta RII) gene transfer. Male C57BL/6 mice were injected with 1.5 x 
      10(8) plaque-forming unit of adenovirus encoding active TGF-beta (AdTGF beta) 
      intraperitoneally. Some mice had been treated with sTGF betaRII or sFlt-1 plasmid 
      injection into skeletal muscle with electroporation 4 days before virus 
      administration. Mice were euthanized at day 14 after virus administration. AdTGF 
      beta induced significant elevation of serum active TGF-beta, caused significant 
      inflammatory response [weight loss, elevation of serum amyloid-P (SAP) and IL-12, 
      increased expression of monocyte chemoattractant protein-1 (MCP-1) mRNA], and 
      induced marked thickening of the peritoneum and collagen deposition. Gene 
      transfer of sFlt-1 reduced the collagen deposition approximately 81% in 
      mesenteric tissue. Treatment with sFlt-1 decreased ICAM-1 and MCP-1 mRNA 
      expression significantly. Significant negative correlation between serum sFlt-1 
      and placental growth factor level was observed, whereas there was no significant 
      negative correlation between sFlt-1 and VEGF. On the other hand, sTGF beta RII 
      treatment enhanced the AdTGF beta-induced inflammation (significant elevation of 
      SAP, TNF-alpha, and IL-12 levels and upregulation of ICAM-1 and MCP-1 mRNA 
      expressions) and failed to prevent collagen deposition. These observations 
      indicate that sFlt-1 gene transfer might be of therapeutic benefit in peritoneal 
      fibrosis.
FAU - Motomura, Y
AU  - Motomura Y
AD  - Intestinal Disease Research Programme, McMaster University, Hamilton, Ontario L8N 
      3Z5, Canada.
FAU - Kanbayashi, H
AU  - Kanbayashi H
FAU - Khan, W I
AU  - Khan WI
FAU - Deng, Y
AU  - Deng Y
FAU - Blennerhassett, P A
AU  - Blennerhassett PA
FAU - Margetts, P J
AU  - Margetts PJ
FAU - Gauldie, J
AU  - Gauldie J
FAU - Egashira, K
AU  - Egashira K
FAU - Collins, S M
AU  - Collins SM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040805
PL  - United States
TA  - Am J Physiol Gastrointest Liver Physiol
JT  - American journal of physiology. Gastrointestinal and liver physiology
JID - 100901227
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Receptors, Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - EC 2.7.10.1 (Vascular Endothelial Growth Factor Receptor-1)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.30 (Receptor, Transforming Growth Factor-beta Type II)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/biosynthesis
MH  - Fibrosis
MH  - *Gene Transfer Techniques
MH  - Inflammation
MH  - Intercellular Adhesion Molecule-1/biosynthesis
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Peritoneum/*pathology
MH  - Protein Serine-Threonine Kinases
MH  - Receptor, Transforming Growth Factor-beta Type II
MH  - Receptors, Transforming Growth Factor beta/*physiology
MH  - Transforming Growth Factor beta
MH  - Up-Regulation
MH  - Vascular Endothelial Growth Factor Receptor-1/*genetics/*physiology
EDAT- 2004/08/07 05:00
MHDA- 2005/03/09 09:00
CRDT- 2004/08/07 05:00
PHST- 2004/08/07 05:00 [pubmed]
PHST- 2005/03/09 09:00 [medline]
PHST- 2004/08/07 05:00 [entrez]
AID - 00186.2004 [pii]
AID - 10.1152/ajpgi.00186.2004 [doi]
PST - ppublish
SO  - Am J Physiol Gastrointest Liver Physiol. 2005 Jan;288(1):G143-50. doi: 
      10.1152/ajpgi.00186.2004. Epub 2004 Aug 5.